Amplicon sequencing data profiling of bacterial community connected with the rhizospheric soil from sunflower plants

Olubukola Oluranti Babalola; Blessing Chidinma Nwachukwu; Ayansina Segun Ayangbenro

doi:10.1016/j.dib.2022.108207

Amplicon sequencing data profiling of bacterial community connected with the rhizospheric soil from sunflower plants

Data Brief. 2022 Apr 26:42:108207. doi: 10.1016/j.dib.2022.108207. eCollection 2022 Jun.

Authors

Olubukola Oluranti Babalola¹, Blessing Chidinma Nwachukwu¹, Ayansina Segun Ayangbenro¹

Affiliation

¹ Food Security and Safety Focus Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, South Africa.

Abstract

This article presents dataset on the bacterial community structure associated with rhizospheric sunflower soils from Lichtenburg, South Africa. The Illumina Miseq sequencing of 16S rRNA gene amplicon unveiled the bacterial community diversities as well as generated metagenomic library from sunflower rhizospheric soils to understand the diversities and distribution. The metagenome contained a total of 41,471 sequences with 45,975 operational taxonomic units (OTUs). Metagenome sequence information is obtainable at NCBI under the Bioproject assigned accession number PRJNA782103. Taxonomic hits distribution from USEARCH analysis at phylum level classification of BN_E discovered predominantly Actinobacteria (33.89%), followed by Proteobacteria (35.45%), Firmicutes (6.45%), Planctomycetes (4.12%), Chloroflexi (4.28%) and Gemmatimonadetes (2.40%). Also, USEARCH assisted analysis of BN_F sample also detected the prevalence of Actinobacteria (45.92%), Proteobacteria (23.23%), Firmicutes (3.84%), Planctomycetes (6.46%), Chloroflexi (4.94%) and Gemmatimonadetes (1.99%), which take part in vital ecological functions and biogeochemical activities needed for plant growth and health.

Keywords: 16S rRNA gene; Illumina MiSeq system; Microbial community; Operational taxonomic units.