Prediction and characterization of a novel hemoglobin-derived mutant peptide (mTgHbP7) from Tegillarca granosa

Xiaoying Zheng; Chun Yuan; Yang Zhang; Shanjie Zha; Fan Mao; Yongbo Bao

doi:10.1016/j.fsi.2022.05.007

Prediction and characterization of a novel hemoglobin-derived mutant peptide (mTgHbP7) from Tegillarca granosa

Fish Shellfish Immunol. 2022 Jun:125:84-89. doi: 10.1016/j.fsi.2022.05.007. Epub 2022 May 7.

Authors

Xiaoying Zheng¹, Chun Yuan², Yang Zhang³, Shanjie Zha⁴, Fan Mao⁵, Yongbo Bao⁶

Affiliations

¹ School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China.
² Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China.
³ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China.
⁴ Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China; Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ninghai, 315604, China.
⁵ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China. Electronic address: maofan@scsio.ac.cn.
⁶ Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China; Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ninghai, 315604, China. Electronic address: baoyongbo@zwu.edu.cn.

PMID: 35537672
DOI: 10.1016/j.fsi.2022.05.007

Abstract

The hemoglobin (Hb) is identified in Tegillarca granosa and its derived peptides have been proved to possess antibacterial activity against gram-positive and gram-negative bacteria. In this study, we identified a series of novel antimicrobial peptides (AMPs) and artificially mutated AMPs derived from subunits of T. granosa Hbs, among which, a mutant T. granosa hemoglobin peptide (mTgHbP) mTgHbP7, was proved to possess predominant antibacterial activity against three bacteria strains (Vibrio alginolyticus, V. parahaemolyticus and Escherichia coli). Besides, mTgHbP7 was predicted to form α-helical structure, which was known to be an important feature of bactericidal AMPs. Furthermore, upon contact with HEK293 cell line, we confirmed that mTgHbP7 had no cytotoxicity to mammalian cell even at a high concentration of 160 μM. Therefore, the findings reported here provide a rationalization for antimicrobial peptide prediction and optimization from mollusk hemoglobin, which will be useful for future development of antimicrobial agents.

Keywords: Antimicrobial peptides; Hemoglobin; Mollusk; Mutant AMPs; Tegillarca granosa.

MeSH terms

Animals
Anti-Bacterial Agents*
Arcidae* / genetics
Arcidae* / microbiology
Escherichia coli
Gram-Negative Bacteria
Gram-Positive Bacteria
HEK293 Cells
Hemoglobins / chemistry
Humans
Mammals
Microbial Sensitivity Tests
Peptides / chemistry

Substances

Anti-Bacterial Agents
Hemoglobins
Peptides