Structural Analysis of Glycans

Stuart M. Haslam; Darón I. Freedberg; Barbara Mulloy; Anne Dell; Pamela Stanley; James H. Prestegard

doi:10.1101/glycobiology.4e.50

Structural Analysis of Glycans

Review

In: Essentials of Glycobiology [Internet]. 4th edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022. Chapter 50.

Authors

Stuart M. Haslam, Darón I. Freedberg, Barbara Mulloy, Anne Dell, Pamela Stanley, James H. Prestegard

Book Editors

Book Affiliations

¹ Distinguished Professor of Medicine and of Cellular & Molecular Medicine, Co-Director, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA
² Professor of Surgery, Beth Israel Deaconess Medical Center, Director, National Center for Functional Glycomics, Harvard Medical School, Boston, Massachusetts, USA
³ Distinguished Professor of Cellular & Molecular Medicine, Co-Director, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA
⁴ Horace W. Goldsmith Foundation Chair, Professor of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, USA
⁵ Sr. Eminent Scholar in Drug Discovery, Georgia Research Alliance William Henry Terry Professor of Biochemistry & Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA
⁶ Professor of Mycology, ETH Zürich, Institute of Microbiology, Department of Biology, Zürich, Switzerland
⁷ Distinguished Research Professor, Georgia Athletics Association Professor in Complex Carbohydrate Research, Professor of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA
⁸ Endowed Chair and Professor, Yabumoto Department of Intractable Disease Research, Research Institute for Microbial Diseases, Immunology Frontier Research Center, Osaka University, Osaka, Japan
⁹ Distinguished Professor of Molecular Sciences, Macquarie University, Sydney, Australia and Principal Research Leader, Institute for Glycomics, Griffith University, Gold Coast, Australia
¹⁰ Professor and Eminent Scholar Emeritus, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA
¹¹ Professor, Departments of Pharmacology and Neuroscience, Johns Hopkins University, Baltimore, Maryland, USA
¹² Director of Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany, Professor, Freie Universität, Berlin, Germany

PMID: 35536953
Bookshelf ID: NBK579945
DOI: 10.1101/glycobiology.4e.50

Excerpt

This chapter surveys techniques for structural characterization of glycans, including composition, linkage, and attachment to aglycones. It covers detection of specific glycan sequences on glycoproteins and cellular surfaces and methods for characterizing structures in three dimensions. The techniques described range from classical chemical detection and characterization of isolated glycan products to sensitive fluorescence methods used in combination with glycan-binding proteins and microscopy on cells and tissues. In addition, nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS), approaches that allow more detailed structural characterization, are discussed.

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