In situ 13CO2 labeling reveals that alpine treeline trees allocate less photoassimilates to roots compared with low-elevation trees

Abstract

Carbon (C) allocation plays a crucial role for survival and growth of alpine treeline trees, however it is still poorly understood. Using in situ 13CO2 labeling, we investigated the leaf photosynthesis and the allocation of 13C labeled photoassimilates in various tissues (leaves, twigs and fine roots) in treeline trees and low-elevation trees. Non-structural carbohydrate concentrations were also determined. The alpine treeline trees (2000 m. a.s.l.), compared with low-elevation trees (1700 m a.s.l.), did not show any disadvantage in photosynthesis, but the former allocated proportionally less newly assimilated C belowground than the latter. Carbon residence time in leaves was longer in treeline trees (19 days) than that in low-elevation ones (10 days). We found an overall lower density of newly assimilated C in treeline trees. The alpine treeline trees may have a photosynthetic compensatory mechanism to counteract the negative effects of the harsh treeline environment (e.g., lower temperature and shorter growing season) on C gain. Lower temperature at treeline may limit the sink activity and C downward transport via phloem, and shorter treeline growing season may result in early cessation of root growth, decreases sink strength, which all together lead to lower density of new C in the sink tissues and finally limit the growth of the alpine treeline trees.

Keywords: 13C pulse labeling; alpine treeline; carbon allocation; non-structural carbohydrates (NSC); photoassimilates; sink activity.