Nitrogen-containing three-dimensional biomass porous carbon materials as an efficient enzymatic biosensing platform for glucose sensing

Baixi Shan; Yanhua Ji; Youbao Zhong; Lai Chen; Shanshan Li; Jie Zhang; Liling Chen; Xuan Liu; Yuan Chen; Nan Yan; Yonggui Song

doi:10.1039/c9ra04008k

Nitrogen-containing three-dimensional biomass porous carbon materials as an efficient enzymatic biosensing platform for glucose sensing

RSC Adv. 2019 Aug 15;9(44):25647-25654. doi: 10.1039/c9ra04008k. eCollection 2019 Aug 13.

Authors

Baixi Shan¹, Yanhua Ji¹, Youbao Zhong¹, Lai Chen¹, Shanshan Li¹, Jie Zhang¹, Liling Chen¹, Xuan Liu¹, Yuan Chen¹, Nan Yan², Yonggui Song¹

Affiliations

¹ Laboratory Animal Science and Technology Center, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine 1688 Meiling Road Nanchang 330006 PR China songyonggui1999@163.com +86 791 87802135 +86 791 87802135.
² National Engineering Research Center for Carbohydrate Synthesis, Jiangxi Normal University Nanchang 330022 PR China.

Abstract

A novel glucose biosensor was developed by immobilizing glucose oxidase (GOD) on a three-dimensional (3D) porous cane vine (wisteria) stem-derived carbon (3D-CVS), which was firstly proposed as novel support material for electrochemical biosensors using loaded biomolecules. Here, an integrated 3D-CVS electrode was fabricated by loading GOD molecule onto a whole piece of 3D-CVS electrode for a glucose biosensor. The morphologies of integrated 3D-CVS and 3D-CVS/GOD electrode were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM results show the 3D macroporous structure of the integrated 3D-CVS electrode. TEM results show that there are some micro-holes and defects in the 3D-CVS electrode. Electrochemical behaviors and electrocatalytic performance of integrated 3D-CVS/GOD electrode were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The effects of pH and scanning rate on the electrochemical response of biosensors have been studied in detail. The glucose biosensor showed a wide linear range from 0.58 μM to 16 mM, with a high sensitivity of 86.17 μA mM^-1 and a low detection limit of 0.19 μM. Furthermore, the glucose biosensor exhibited high selectivity, good repeatability and nice stability.