Ozone treatment at a suitable concentration can improve the antioxidant capacity of postharvest fruits. However, few studies have examined the antioxidant bioactive compounds in ozone-treated postharvest strawberries, especially in relation to proteomics. In this study, the total phenol content (TPC), total flavonoid content (TFC), and total anthocyanin content (TAC) were used as the main antioxidant compound indicators and unlabeled proteomics was used to study the metabolism of phenylpropanoids in postharvest strawberries (Jingtaoxiang) treated with different concentrations of ozone (0, 1, 3, and 5 ppm) throughout the duration of storage. The results showed that the postharvest strawberries treated with 5 ppm ozone concentration exhibited improved accumulation of total phenols, flavonoids and anthocyanins in the antioxidant bioactive compounds, which was beneficial to the expression of phenylpropanoid metabolism-related proteins over the whole storage period compared with the other three groups. The results of proteomics were consistent with the changes in the key metabolites of phenylpropanoids, which indicated that ozone treatment at a suitable concentration aids the accumulation of TPC, TAC and TFC by promoting the key proteins associated with phenylpropanoid metabolism.
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