Bioassay-guided purification of sesquiterpenoids from the fruiting bodies of Fomitopsis pinicola and their anti-inflammatory activity

Shih-Huang Tai; Ping-Chung Kuo; Ching-Che Hung; Ying-Hsuan Lin; Tsong-Long Hwang; Sio Hong Lam; Daih-Huang Kuo; Jin-Bin Wu; Hsin-Yi Hung; Tian-Shung Wu

doi:10.1039/c9ra05899k

Bioassay-guided purification of sesquiterpenoids from the fruiting bodies of Fomitopsis pinicola and their anti-inflammatory activity

RSC Adv. 2019 Oct 23;9(59):34184-34195. doi: 10.1039/c9ra05899k.

Authors

Shih-Huang Tai¹, Ping-Chung Kuo², Ching-Che Hung³, Ying-Hsuan Lin⁴, Tsong-Long Hwang⁵, Sio Hong Lam², Daih-Huang Kuo⁶, Jin-Bin Wu⁷, Hsin-Yi Hung², Tian-Shung Wu^{2

6}

Affiliations

¹ Departments of Surgery and Anesthesiology, Institute of Biomedical Engineering, National Cheng Kung University, Medical Center and Medical School Tainan 701 Taiwan.
² School of Pharmacy, College of Medicine, National Cheng Kung University Tainan 70101 Taiwan tswu@mail.ncku.edu.tw z10308005@email.ncku.edu.tw +886-6-2740552 +886-6-2373149 +886-6-2747538 +886-6-2353535 ext. 6803.
³ Department of Chemistry, National Cheng Kung University Tainan 70101 Taiwan.
⁴ Department of Environmental Sciences, University of California Riverside California 92521 USA.
⁵ Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Research Center for Industry of Human Ecology, Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Department of Anesthesiology, Chang Gung Memorial Hospital Taoyuan 333 Taiwan.
⁶ Department of Pharmacy, College of Pharmacy and Health Care, Tajen University Pingtung 907 Taiwan.
⁷ Jen-Li Biotechnology Company Taichung Taiwan.

Abstract

Twelve undescribed sesquiterpenoids, fomitopins A-L (1-12), were isolated via bioassay-guided purification from the bracket fungus Fomitopsis pinicola (Sw.) P. Karst, and this fungus have been reported to exhibit anti-microbial and anti-inflammatory activities. The structures of 1-12 were elucidated by spectroscopic and spectrometric analyses and their absolute configurations were further confirmed by ECD simulations. Ten isolated compounds were evaluated for their anti-inflammatory potential and compound 11 exhibited the most significant inhibition of superoxide anion generation and elastase release with IC₅₀ values of 0.81 ± 0.15 and 0.74 ± 0.12 μM. These newly purified sesquiterpenoids could be potential candidates for further anti-inflammatory studies.