A novel ratiometric fluorescent probe for the selective determination of HClO based on the ESIPT mechanism and its application in real samples

Jingrui Li; Aijun Gong; Guoqing Shi; Chengwen Chai

doi:10.1039/c9ra04569d

A novel ratiometric fluorescent probe for the selective determination of HClO based on the ESIPT mechanism and its application in real samples

RSC Adv. 2019 Sep 30;9(53):30943-30951. doi: 10.1039/c9ra04569d. eCollection 2019 Sep 26.

Authors

Jingrui Li^{1

2}, Aijun Gong^{1

2}, Guoqing Shi¹, Chengwen Chai¹

Affiliations

¹ School of Chemistry and Biological Engineering, University of Science and Technology Beijing Beijing 100083 China Gongaijun5661@ustb.edu.cn +86-010-62334071 +86-010-82375661.
² Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline Materials, University of Science and Technology Beijing Beijing 100083 China.

Abstract

Based on the ESIPT fluorescence mechanism, herein, a novel ratiometric fluorescent probe was designed and synthesized for the detection of HClO. The reaction site of diaminomaleonitrile at the ortho-position of the phenolic hydroxyl group made the probe exhibit a ratiometric fluorescence response towards hypochlorous acid (HClO). The specific sensing mechanism was verified via MS, HPLC and ¹H NMR spectroscopy. Moreover, the probe showed excellent performance with high sensitivity and good selectivity towards HClO in the presence of other reactive oxygen species. In addition, the probe was successfully applied to detect HClO spiked in tap water, river water and diluted human serum with good recoveries.