A fatal case of haemorrhagic fever with renal syndrome in Kursk Region, Russia, caused by a novel Puumala virus clade

Ekaterina Blinova; Andrei Deviatkin; Svetlana Kurashova; Maria Balovneva; Irina Volgina; Anna Valdokhina; Victoria Bulanenko; Yulia Popova; Alla Belyakova; Tamara Dzagurova

doi:10.1016/j.meegid.2022.105295

A fatal case of haemorrhagic fever with renal syndrome in Kursk Region, Russia, caused by a novel Puumala virus clade

Infect Genet Evol. 2022 Aug:102:105295. doi: 10.1016/j.meegid.2022.105295. Epub 2022 May 5.

Affiliations

¹ Federal Budget Institution of Science "Central Research Institute of Epidemiology" of The Federal Service on Customers' Rights Protection and Human Well-being Surveillance, Moscow 111123, Russian Federation; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow 108819, Russian Federation. Electronic address: blinova.e@cmd.su.
² The National Medical Research Center for Endocrinology, Moscow 117036, Russian Federation.
³ Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow 108819, Russian Federation.
⁴ Federal Budgetary Healthcare Institution "Center for Hygiene and Epidemiology in the Kursk Region", Kursk 305000, Russian Federation.
⁵ Federal Budget Institution of Science "Central Research Institute of Epidemiology" of The Federal Service on Customers' Rights Protection and Human Well-being Surveillance, Moscow 111123, Russian Federation.

PMID: 35526822
DOI: 10.1016/j.meegid.2022.105295

Abstract

Haemorrhagic fever with renal syndrome (HFRS) is the most widespread natural-focal human disease in the Russian Federation. In this study, we report virological assessment of a fatal case of HFRS-PUUV (Puumala virus) in the Kursk Region. The infection caused severe multiorgan failure and the maximum viral load was detected in the tissue of the spleen. Viral sequences were obtained from the patient's autopsy material and lung tissues of bank voles captured in the region. These sequences formed a new clade in the PUUV phylogenetic tree, an outgroup to all known Russian (RUS) lineage sequences. On the other hand viruses collected in the Kursk Region grouped with the RUS lineage and are separated from all other PUUV linages. We propose to nominate this novel group as W-RUS as the identified viruses were collected near the western Russian boundary. The recombination signals between their ancestors and RUS lineage representatives from the Volga region were revealed. The strain Samara_94/CG/2005 suggestively emerged as the result of reassortment between the ancestors of W-RUS and DTK-Ufa-97.

Keywords: Bank vole; HFRS; Hantaviruses; Lineage; PUUV; Recombination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arvicolinae
Hemorrhagic Fever with Renal Syndrome*
Humans
Phylogeny
Puumala virus* / genetics
Russia
Viruses*