PLAAT1 promotes p53 degradation via autophagy-lysosome pathway in zebrafish

Xin Zhao; Wenji Huang; Jiahong Guo; Ning Ji; Jianhua Feng; Yanjie Shi; Kangyong Chen; Jun Zou

doi:10.1016/j.fsi.2022.05.001

PLAAT1 promotes p53 degradation via autophagy-lysosome pathway in zebrafish

Fish Shellfish Immunol. 2022 Jun:125:48-53. doi: 10.1016/j.fsi.2022.05.001. Epub 2022 May 5.

Authors

Xin Zhao¹, Wenji Huang¹, Jiahong Guo¹, Ning Ji¹, Jianhua Feng¹, Yanjie Shi¹, Kangyong Chen¹, Jun Zou²

Affiliations

¹ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
² Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: jzou@shou.edu.cn.

PMID: 35526800
DOI: 10.1016/j.fsi.2022.05.001

Abstract

PLAAT1 belongs to the PLAAT family and plays regulatory roles in cell growth, tumor suppression and phospholipid metabolism. However, whether PLAAT1 is involved in p53 mediated signaling has not been investigated. Here, we report that PLAAT1 promotes degradation of p53 in zebrafish. We found that the plaat1 gene was constitutively expressed in tissues including liver, kidney, spleen, intestine, eye and brain, with relative higher expression levels detected in the brain and eye. Overexpression of plaat1 led to inhibition of p53 and tnfα mRNA expression. Furthermore, it was shown that PLAAT1 interacted with p53 to facilitate p53 degradation via autophagy-lysosome dependent pathway. Our work indicates that PLAAT1 is involved in the interplay between p53 mediated cellular responses and autophagy.

Keywords: Apoptosis; Autophagy; Phospholipase A/acyltransferases; Protein interaction; Zebrafish; p53.

MeSH terms

Animals
Apoptosis
Autophagy / genetics
Lysosomes / metabolism
Signal Transduction
Tumor Suppressor Protein p53* / genetics
Tumor Suppressor Protein p53* / metabolism
Zebrafish* / genetics
Zebrafish* / metabolism

Substances

Tumor Suppressor Protein p53