Platelet rich fibrin containing nanofibrous dressing for wound healing application: Fabrication, characterization and biological evaluations

Marjan Mirhaj; Mohamadreza Tavakoli; Jaleh Varshosaz; Sheyda Labbaf; Farnoosh Jafarpour; Parvin Ahmaditabar; Saeideh Salehi; Nafise Kazemi

doi:10.1016/j.msec.2021.112541

Platelet rich fibrin containing nanofibrous dressing for wound healing application: Fabrication, characterization and biological evaluations

Biomater Adv. 2022 Mar:134:112541. doi: 10.1016/j.msec.2021.112541. Epub 2021 Nov 17.

Authors

Marjan Mirhaj¹, Mohamadreza Tavakoli¹, Jaleh Varshosaz², Sheyda Labbaf³, Farnoosh Jafarpour⁴, Parvin Ahmaditabar⁵, Saeideh Salehi⁶, Nafise Kazemi⁶

Affiliations

¹ Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
² Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic address: varshosaz@pharm.mui.ac.ir.
³ Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: s.labbaf@iut.ac.ir.
⁴ Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
⁵ Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
⁶ Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.

PMID: 35525762
DOI: 10.1016/j.msec.2021.112541

Abstract

Recently, nanofibrous structures have shown great potential for a wide range of medical applications. The aim of the current study was to evaluate the wound healing process using Polycaprolactone/Keratin/Platelet-rich fibrin (PCL/Kr/PRF) fibrous scaffold fabricated through electrospinning process. A range of techniques were utilized to fully characterize the chemical, physical and biological properties of the resultant structure. Results revealed that by the addition of only 0.5%w/v PRF to PCL/Kr (PCL/Kr/0.5PRF) sample, the fibers diameter decreased from 193.93 ± 64.80 nm to 65.98 ± 14.03 nm, and the stress at break demonstrated a 18.27% increase in comparison to the PCL sample (from 2.90 ± 0.80 MPa to 3.43 ± 0.90 MPa). The PCL/Kr/0.5PRF scaffold showed more antibacterial activity against gram-positive and gram-negative bacteria than PCL/Kr sample. Based on enzyme-linked immunosorbent assays, the PCL/Kr/0.5PRF sample revealed an independent release of VEGF and PDGF for 7 days. Cell viability studies demonstrated non-cytotoxic nature of PRF-containing dressings. Also, chorioallantoic membrane (CAM) assay was performed to evaluate the angiogenic potential of the wound dressings. The in vivo assessments also showed that PCL/Kr/0.5PRF accelerated the wound healing process in terms of collagen deposition and the formation of skin appendages which was comparable to the normal skin. Overall, the data presented in this study greatly suggest that the PCL/Kr/0.5PRF wound dressing could be a suitable candidate for wound healing and skin regeneration.

Keywords: Electrospinning; Keratin; Nanofiber; Platelet-rich fibrin; Wound healing.

MeSH terms

Anti-Bacterial Agents / chemistry
Bandages
Gram-Negative Bacteria
Gram-Positive Bacteria
Nanofibers* / chemistry
Platelet-Rich Fibrin*
Wound Healing

Substances

Anti-Bacterial Agents