Mutagenesis analysis of GMN motif in Arabidopsis thaliana Mg2+ transporter MRS2-1

Xiaoyu Yang; Natsuko I Kobayashi; Yoshiki Hayashi; Koichi Ito; Yoshitaka Moriwaki; Tohru Terada; Kentaro Shimizu; Motoyuki Hattori; Ren Iwata; Hisashi Suzuki; Tomoko M Nakanishi; Keitaro Tanoi

doi:10.1093/bbb/zbac064

Mutagenesis analysis of GMN motif in Arabidopsis thaliana Mg2+ transporter MRS2-1

Biosci Biotechnol Biochem. 2022 Jun 25;86(7):870-874. doi: 10.1093/bbb/zbac064.

Authors

Xiaoyu Yang¹, Natsuko I Kobayashi¹, Yoshiki Hayashi², Koichi Ito², Yoshitaka Moriwaki^{3

4}, Tohru Terada^{3

4}, Kentaro Shimizu^{3

4}, Motoyuki Hattori⁵, Ren Iwata⁶, Hisashi Suzuki⁷, Tomoko M Nakanishi^{1

8}, Keitaro Tanoi¹

Affiliations

¹ Laboratory of Radio Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
² Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
³ Bioinformation Engineering Laboratory, Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
⁴ Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
⁵ State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Neurobiology, School of Life Sciences, Fudan University, Shanghai, China.
⁶ Division of Radiopharmaceutical Chemistry, Cyclotron and Radioisotope Center, Tohoku University, Sendai, Japan.
⁷ National Institute for Quantum Science and Technology, Chiba, Japan.
⁸ Hoshi University, Tokyo, Japan.

PMID: 35524690
DOI: 10.1093/bbb/zbac064

Abstract

Magnesium is an important nutrient for plants, but much is still unknown about plant Mg2+ transporters. Combining with the structural prediction of AlphaFold2, we used mutagenesis and 28Mg uptake assay to study the highly conserved "GMN" motif of Arabidopsis thaliana MRS2-1 (AtMRS2-1) transporter. We demonstrated that the glycine and methionine in GMN motif are essential for AtMRS2-1 to transport Mg2+.

Keywords: 28Mg; GMN motif; magnesium transporter; plant nutrition.

MeSH terms

Arabidopsis Proteins* / metabolism
Arabidopsis* / genetics
Arabidopsis* / metabolism
Cation Transport Proteins* / genetics
Magnesium / metabolism
Mutagenesis

Substances

Arabidopsis Proteins
Cation Transport Proteins
Magnesium

Abstract

MeSH terms

Substances

Grants and funding