Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species

Marcin Nowicki; Matthew L Huff; Margaret E Staton; Robert N Trigiano

doi:10.1007/s13353-022-00699-8

Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species

J Appl Genet. 2022 Sep;63(3):463-467. doi: 10.1007/s13353-022-00699-8. Epub 2022 May 6.

Authors

Marcin Nowicki¹, Matthew L Huff², Margaret E Staton², Robert N Trigiano²

Affiliations

¹ Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA. mnowicki@utk.edu.
² Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN, USA.

Abstract

The complete chloroplast genome of Pyrus calleryana (GenBank OM541581.1) was developed by de novo assembly from whole-genome sequencing data. Reference-guided (P. phaeocarpa) read mapping and assembly were followed by annotation and phylogenetic comparisons. The 159,965 bp P. calleryana chloroplast genome represented 36.56% GC content with a classical quadripartite architecture and two inverted repeats regions (IRs; each 26,392 bp) separating the large single-copy region (LSC; 87,942 bp) and the small single-copy region (SSC; 19.239 bp). In total, 125 unique features were annotated in that genome, including 83 protein coding genes, 38 tRNA coding genes, and 4 rRNA coding genes. Phylogenetic analyses based on the whole chloroplast genome sequences placed the P. calleryana among other Rosaceae plants, specifically among the Asian species of Pyrus.

Keywords: Callery pear; Pears; Plastid genome annotation; Plastome; Reference-guided assembly.

MeSH terms

Base Composition
Genome, Chloroplast*
Phylogeny
Pyrus* / genetics
Whole Genome Sequencing