Inducing Global Expression of Actinobacterial Biosynthetic Gene Clusters

Abstract

Bacteria produce an impressive array of bioactive specialized metabolites, with Streptomyces (and the actinobacteria more generally) being unusually diverse and prolific producers. However, the biosynthetic potential of these organisms has yet to be fully explored, as many of the biosynthetic gene clusters that direct the synthesis of these natural products are transcriptionally silent under laboratory growth conditions. Here, we describe strategies that can be employed to broadly stimulate the expression of biosynthetic gene clusters in Streptomyces and their relatives, follow the transcription of these genes, and assess the antimicrobial activity of the resulting molecules.

Keywords: Antibiotic; Bioassay; Biosynthetic gene cluster; Gene expression; Genetic engineering; Streptomyces; Transcription regulator.