Enhanced CO2 reduction and acetate synthesis in autotrophic biocathode by N-Hexanoyl-L-homoserine lactone (C6HSL)-based quorum-sensing regulation

Abstract

The aim of this study was to investigate the ecological role of quorum-sensing signaling molecule on the autotrophic biocathode for CO₂ reduction and acetate synthesis. As a typical quorum-sensing signaling molecule, N-Hexanoyl-L-homoserine lactone (C6HSL) was used to regulate the construction of cathode biofilm. Results showed that the maximum acetate production from CO₂ reduction improved by 94.8%, and the maximum Faraday efficiency of the microbial electrosynthesis system enhanced by 71.7%, with the regulation of C6HSL. Electrochemical analyses indicated that higher electrochemical activity and lower charge resistance of biocathode were obtained with C6HSL than without C6HSL. Confocal laser scanning microscopy and electron inhibitor experiment suggested that exogenous C6HSL increased living biomass in the biofilm and facilitated the electron transfer pathway related to NADH dehydrogenase-CoQ and proton motive force. With the C6HSL regulation, the relative abundance of hydrogen producers (e.g., Desulfovibrio and Desulfomicrobium) increased, contributing to the improved performance of autotrophic biocathode.

Keywords: Acetate synthesis; Autotrophic biocathode; Electron transfer pathway; Microbial community; Quorum sensing.