LF-NMR/MRI Determination of Different 6-Benzylaminopurine Concentrations and Their Effects on Soybean Moisture

Front Plant Sci. 2022 Apr 18:13:885804. doi: 10.3389/fpls.2022.885804. eCollection 2022.

Abstract

In this study, we aimed to clarify the distribution and dynamics of water in the Xudou 20 soybean cultivar post-germination after culturing plants with various concentrations of 6-benzylaminopurine (6-BA). Low-field nuclear magnetic resonance and magnetic resonance imaging (LF-NMR/MRI), as well as principal component analysis (PCA), were used for the investigation. Results showed that low concentrations of 6-BA promoted soybean germination and high concentrations inhibited soybean germination, with 5 mg/l of 6-BA producing the most optimal conditions for growth. Moreover, the T 22 determination of weakly bound water increased with increasing 6-BA concentration, and the PCA effectively distinguished soybeans cultured at different 6-BA concentrations. This study provides a method for the rapid detection of 6-BA concentration in bean sprouts and provides theoretical support and bean sprout quality assessment.

Keywords: 6-benzylaminopurine; magnetic resonance imaging; nuclear magnetic resonance; principal component analysis; water dynamics.