Optimization of photo-biomodulation therapy for wound healing of diabetic foot ulcers in vitro and in vivo

Qianqian Chen; Jichun Yang; Huijuan Yin; Yingxin Li; Haixia Qiu; Ying Gu; Hua Yang; Dong Xiaoxi; Shi Xiafei; Bochen Che; Hongxiao Li

doi:10.1364/BOE.451135

Optimization of photo-biomodulation therapy for wound healing of diabetic foot ulcers in vitro and in vivo

Biomed Opt Express. 2022 Mar 25;13(4):2450-2466. doi: 10.1364/BOE.451135. eCollection 2022 Apr 1.

Authors

Qianqian Chen^{1

2

3}, Jichun Yang^{1

3}, Huijuan Yin¹, Yingxin Li¹, Haixia Qiu⁴, Ying Gu⁴, Hua Yang⁵, Dong Xiaoxi¹, Shi Xiafei¹, Bochen Che¹, Hongxiao Li¹

Affiliations

¹ Laboratory of Laser Medicine, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China.
² National Research Center for Rehabilitation Technical Aids, Beijing Key Laboratory of Rehabilitation Technical Aids for Old-Age Disability, Key Laboratory of Human Motion Analysis and Rehabilitation Technology of the Ministry of Civil Affairs, Beijing 100176, China.
³ Equal contributors.
⁴ Department of Laser Medicine, Chinese PLA General Hospital, Beijing 100853, China.
⁵ Semiconductor Lighting Technology Research and Development Center, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

Abstract

Unclear optical parameters make photo-biomodulation (PBM) difficult to implement in diabetic foot ulcer (DFU) clinically. Here, 12 wavelengths (400-900 nm) were used to conduct PBM to heal DFU wounds in vitro and in vivo. PBM at 10 mW/cm² and 0.5-4 J/cm² with all 12 wavelengths promoted proliferation of diabetic wound cells. In a mimic DFU (mDFU) rat model, PBM (425, 630, 730, and 850 nm, and a combination light strategy) promoted mDFU healing. The positive cell proliferation, re-epithelialization, angiogenesis, collagen synthesis, and inflammation were possible mechanisms. The combination strategy had the best effect, which can be applied clinically.