Noninvasive assessment of liver function reserve with fluorescent dosimetry of indocyanine green

Pei-Chun Wu; Lun-Zhang Guo; Shan Yu; Ning Zeng; Yu-Cheng Liu; Jia Yu; Zhiming Zhang; Ke Lu; Liangyu Sun; Chunfei Wang; Yu-Han Chang; Yin-Lin Lu; Yu-Fang Shen; Sheng Tai; Yueh-Hsun Chuang; Ja-An Annie Ho; Kai-Wen Huang; Yao-Ming Wu; Tzu-Ming Liu

doi:10.1364/BOE.446749

Noninvasive assessment of liver function reserve with fluorescent dosimetry of indocyanine green

Biomed Opt Express. 2022 Mar 10;13(4):1995-2005. doi: 10.1364/BOE.446749. eCollection 2022 Apr 1.

Authors

Pei-Chun Wu^{1

2

3

4}, Lun-Zhang Guo^{2

4}, Shan Yu^{1

5

4}, Ning Zeng^{6

7

4}, Yu-Cheng Liu¹, Jia Yu¹, Zhiming Zhang¹, Ke Lu¹, Liangyu Sun¹, Chunfei Wang¹, Yu-Han Chang^{2

3}, Yin-Lin Lu^{1

3}, Yu-Fang Shen^{8

9}, Sheng Tai¹⁰, Yueh-Hsun Chuang¹¹, Ja-An Annie Ho¹², Kai-Wen Huang¹³, Yao-Ming Wu¹⁴, Tzu-Ming Liu¹

Affiliations

¹ Institute of Translational Medicine, Faculty of Health Sciences & Ministry of Education Frontiers Science Center for Precision Oncology, University of Macau, Taipa, Macau, China.
² Department of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
³ Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
⁴ Contributed equally.
⁵ Department of Pathology, The Secondary Affiliated Hospital of Harbin Medical University, Harbin 150080, China.
⁶ First Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
⁷ Guangdong Provincial Clinical and Engineering Technology Center of Digital Medicine, Guangzhou 510280, China.
⁸ 3D Printing Medical Research Institute, Asia University, Taichung 41354, Taiwan.
⁹ Department of Bioinformatics and Medical Engineering, Asia University, Taichung 41354, Taiwan.
¹⁰ Department of Hepatopancreatobiliary Surgery, The Secondary Affiliated Hospital of Harbin Medical University, Harbin 150080, China.
¹¹ Department of Anesthesiology, National Taiwan University Hospital, Taipei 10002, Taiwan.
¹² Bioanalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science & Technology, National Taiwan University, Taipei 10617, Taiwan.
¹³ Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei 10002, Taiwan.
¹⁴ Department of Surgery, National Taiwan University Hospital and College of Medicine, Taipei 10002, Taiwan.

Abstract

Using in vivo multiphoton fluorescent dosimetry, we demonstrate that the clearance dynamics of Indocyanine Green (ICG) in the blood can quickly reveal liver function reserve. In normal rats, the ICG retention rate was below 10% at the 15-minute post-administration; While in the rat with severe hepatocellular carcinoma (HCC), the 15-minute retention rate is over 40% due to poor liver metabolism. With a 785 nm CW laser, the fluorescence dosimeter can evaluate the liver function reserve at a 1/10 clinical dosage of ICG without any blood sampling. In the future, this low-dosage ICG 15-minute retention dosimetry can be applied for the preoperative assessment of hepatectomy or timely perioperative examination.