Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR

Sivaranjan Uppala; Ayelet Gamliel; Gal Sapir; Jacob Sosna; J Moshe Gomori; Rachel Katz-Brull

doi:10.1039/d0ra08022e

Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR

RSC Adv. 2020 Nov 11;10(67):41197-41201. doi: 10.1039/d0ra08022e. eCollection 2020 Nov 9.

Authors

Sivaranjan Uppala¹, Ayelet Gamliel¹, Gal Sapir¹, Jacob Sosna¹, J Moshe Gomori¹, Rachel Katz-Brull¹

Affiliation

¹ Department of Radiology, Hadassah Medical Center, Hebrew University of Jerusalem, The Faculty of Medicine Jerusalem Israel rkb@hadassah.org.il.

Abstract

A hyperpolarised-NMR acquisition approach that is sensitive to the process of glucose-6-phosphate anomerization is presented. Using selective depolarisation of one of the anomer's signals, it is possible to observe the replenishing of this signal due to the fast anomeric exchange of this compound. The forward to reverse reaction rate constants ratio was ca. 1.6.