A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR-Cas9 modified catalytic hairpin assembly

Yang Liu; Shihong Li; Likun Zhang; Qian Zhao; Nuo Li; Yuxin Wu

doi:10.1039/d0ra03603j

A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR-Cas9 modified catalytic hairpin assembly

RSC Adv. 2020 Jul 28;10(47):28037-28040. doi: 10.1039/d0ra03603j. eCollection 2020 Jul 27.

Authors

Yang Liu¹, Shihong Li¹, Likun Zhang¹, Qian Zhao¹, Nuo Li¹, Yuxin Wu¹

Affiliation

¹ Department of Clinical Laboratory, The Third Affiliated Hospital of Jinzhou Medical University Jinzhou Liaoning Province 121000 China jykly43@163.com.

Abstract

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR-Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR-Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection and in situ miR-21 imaging.