Supermolecule-assisted imaging of low-molecular-weight quaternary-ammonium compounds by MALDI-MS of their non-covalent complexes with cucurbit[7]uril

Di Chen; Jun Han; Juncong Yang; David Schibli; Zhenzhong Zhang; Christoph H Borchers

doi:10.1039/d0ra04604c

Supermolecule-assisted imaging of low-molecular-weight quaternary-ammonium compounds by MALDI-MS of their non-covalent complexes with cucurbit[7]uril

RSC Adv. 2020 Sep 15;10(56):34261-34265. doi: 10.1039/d0ra04604c. eCollection 2020 Sep 10.

Authors

Di Chen^{1

2}, Jun Han^{1

3}, Juncong Yang¹, David Schibli¹, Zhenzhong Zhang², Christoph H Borchers^{4

5

6

7}

Affiliations

¹ University of Victoria - Genome British Columbia Proteomics Centre Victoria BC V8Z 7X8 Canada HanJun@proteincentre.com.
² School of Pharmaceutical Sciences, Zhengzhou University Zhengzhou 450001 China.
³ Division of Medical Sciences, University of Victoria Victoria BC V8P 5C2 Canada.
⁴ Department of Biochemistry and Microbiology, University of Victoria Victoria BC V8P 5C2 Canada.
⁵ Gerald Bronfman Department of Oncology, Jewish General Hospital, McGill University Montreal Quebec H3T 1E2 Canada christoph.borchers@mcgill.ca.
⁶ Segal Cancer Proteomics Centre, Lady Davis Institute, Jewish General Hospital, McGill University Montreal Quebec H3T 1E2 Canada.
⁷ Center for Computational and Data-Intensive Science and Engineering, Skolkovo Institute of Science and Technology Moscow 121205 Russia.

Abstract

Cucurbit[7]uril was used to form non-covalent complexes with low-molecular-weight quaternary-ammonium compounds for their indirect analysis by MALDI-MS. By shifting the ion signals to a higher and interference-free mass region, the distributions of neurine, choline, and phosphocholine in rat brain were visualized by MALDI imaging with high selectivity and good sensitivity.