Detection of signal molecules in living plants is of great relevance for precision farming. In this work, to establish a more effective method for monitoring salicylic acid (SA) in the leaves of living plants, a ratiometric electrochemical sensor was fabricated based on a Cu metal-organic framework (Cu-MOF) and carbon black (CB) composite. The Cu-MOF and CB composite was used to catalyze SA oxidation. Ratiometric oxidation current peak intensities I SA/I Cu-MOFs were used as the response signal for SA. I SA/I Cu-MOFs linearly enhanced with the increase of SA concentration, together with low limits of detection (12.50 μM). Moreover, our sensor is fabricated on a screen-printed electrode (SPE), which is especially suitable for applying to the flat leaves of plants. Using this sensor, the SA level in the leaves of cucumber seedlings was monitored in vivo under salt stress. The proposed sensor is accurate, reliable and practical. This is the first report for developing a ratiometric electrochemical sensor for detecting SA in living plants. Our work can also provide a strategy for in vivo studies on the leaves of plants.
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