miRNA-199a-5p/SLC2A1 axis regulates glucose metabolism in non-small cell lung cancer

Yikun Xu; Binshu Chai; Xianyi Wang; Zong Wu; Zhitao Gu; Xiaomin Liu; Yiqi Zhao; Tangbing Chen; Zhongliang Ma; Qiangling Sun

doi:10.7150/jca.67990

miRNA-199a-5p/SLC2A1 axis regulates glucose metabolism in non-small cell lung cancer

J Cancer. 2022 Apr 18;13(7):2352-2361. doi: 10.7150/jca.67990. eCollection 2022.

Authors

Yikun Xu¹, Binshu Chai¹, Xianyi Wang¹, Zong Wu¹, Zhitao Gu², Xiaomin Liu^{1

3}, Yiqi Zhao¹, Tangbing Chen², Zhongliang Ma¹, Qiangling Sun^{2

4}

Affiliations

¹ Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, 200444, China.
² Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, China.
³ Shanghai New Tobacco Product Research Institute, Shanghai, 201315, China.
⁴ Thoracic Cancer institute, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, China.

Abstract

Lung cancer is acknowledged as a common cancer with high morbidity and mortality. MicroRNAs (miRNAs), kind of non-coding single-stranded RNA molecules, can be used in cancer clinical treatments. In this research, miR-199a-5p was seen lowly expressed in NSCLC sera samples. miR-199a-5p suppressed the cell proliferation, migration and arrested cell cycle in NSCLC cell lines. The results showed that SLC2A1 (glucose transporter 1, GLUT1) was a direct target of miR-199a-5p. Downregulation of SLC2A1 could not only inhibit cell proliferation, migration and cell cycle, but also promote cell apoptosis. The data suggests that miR-199a-5p can inhibit glucose metabolism in NSCLC by targeting SLC2A1.This study proves that miR-199a-5p / SLC2A1 can play an essential role in the development of NSCLC by targeting SLC2A1. It puts forward a new approach for clinical treatments of NSCLC.

Keywords: GLUTs; NSCLC; SLC2A1/GLUT1; miR-199a-5p; non-coding RNAs.