Pullulan nanofibers containing the antimicrobial palindromic peptide LfcinB (21-25)Pal obtained via electrospinning

Julieth Tatiana Román; Carlos Alberto Fuenmayor; Carlos Mario Zuluaga Dominguez; Dianney Clavijo-Grimaldo; Martha Acosta; Javier Eduardo García-Castañeda; Ricardo Fierro-Medina; Zuly Jenny Rivera-Monroy

doi:10.1039/c9ra03643a

Pullulan nanofibers containing the antimicrobial palindromic peptide LfcinB (21-25)_Pal obtained via electrospinning

RSC Adv. 2019 Jul 1;9(35):20432-20438. doi: 10.1039/c9ra03643a. eCollection 2019 Jun 25.

Authors

Julieth Tatiana Román¹, Carlos Alberto Fuenmayor², Carlos Mario Zuluaga Dominguez³, Dianney Clavijo-Grimaldo⁴, Martha Acosta⁵, Javier Eduardo García-Castañeda¹, Ricardo Fierro-Medina¹, Zuly Jenny Rivera-Monroy¹

Affiliations

¹ Science Faculty, Universidad Nacional de Colombia Av. carrera 30 no. 45-03 Bogotá Zip code 111321 Colombia zjriveram@unal.edu.co +57 1 3165000 ext. 14436.
² Instituto de Ciencia y Tecnología de alimentos (ICTA), Universidad Nacional de Colombia Av. carrera 30 no. 45-03 Bogotá Zip code 111321 Colombia.
³ Agricultural Sciences Faculty, Universidad Nacional de Colombia Av. carrera 30 no. 45-03 Bogotá Zip code 111321 Colombia.
⁴ Medicine Faculty, Universidad Nacional de Colombia Av. carrera 30 no. 45-03 Bogotá Zip code 111321 Colombia.
⁵ Servicio Nacional de Aprendizaje (SENA) Technopark Calle 54 No. 10-39 Zip code 110231 Bogotá Colombia.

Abstract

Electrospinning technology is useful for making ultrafine drug-eluting fibers for the clinical treatment of wounds. We show the incorporation of an antimicrobial LfcinB-derived peptide into Pullulan nanofibers. The palindromic peptide LfcinB (21-25)_Pal: RWQWRWQWR was synthesized, purified, and characterized by means of the RP-HPLC and MALDI-TOF MS methods. The peptide's antibacterial activity against the E. coli ATCC 25922 strain was evaluated, and the peptide LfcinB (20-25)_Pal exhibited significant antibacterial activity. Nanofibers were obtained by electrospinning a Pullulan or Pullulan-LfcinB (21-25)_Pal solution. The obtained nanofibers were characterized via microscopy (AFM and SEM) and RP-HPLC chromatography. The peptide incorporation efficiency was 31%. The Pullulan-LfcinB (21-25)_Pal nanofibers were soluble in water, and the peptide was liberated immediately. The Pullulan-LfcinB (21-25)_Pal nanofibers exhibited the same antibacterial activity against E. coli strain as the free peptide LfcinB (21-25)_Pal. The results suggest that Pullulan-LfcinB (21-25)_Pal nanofibers could be considered for designing and developing antibacterial wound dressings.