Rapid detection of the emerging tick-borne Tamdy virus by TaqMan-based real-time reverse transcription PCR

Mingxue Cui; Hong Zhou; Bin Zhang; Michael J Carr; Moujian Guo; Weifeng Shi

doi:10.1016/j.jviromet.2022.114538

Rapid detection of the emerging tick-borne Tamdy virus by TaqMan-based real-time reverse transcription PCR

J Virol Methods. 2022 Jul:305:114538. doi: 10.1016/j.jviromet.2022.114538. Epub 2022 May 2.

Authors

Mingxue Cui¹, Hong Zhou¹, Bin Zhang², Michael J Carr³, Moujian Guo¹, Weifeng Shi⁴

Affiliations

¹ Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China.
² College of Life Sciences & Technology, Inner Mongolia Normal University, Hohhot, Inner Mongolia 010022, China.
³ National Virus Reference Laboratory, School of Medicine, University College Dublin, Belfield, Dublin 4, Ireland; International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, N20 W10 Kita-ku, Sapporo 001-0020, Japan.
⁴ Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China; School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong 271000, China. Electronic address: shiwf@ioz.ac.cn.

PMID: 35513136
DOI: 10.1016/j.jviromet.2022.114538

Abstract

Tamdy virus (TAMV) is an emerging zoonotic tick-borne arbovirus in the genus Orthonairovirus. Reports of human infections with TAMV have been increasing and development of a rapid detection assay is thus urgently required. In the present study, singleplex and dual-target real-time reverse transcription PCR (qRT-PCR) assays were established for the detection of TAMV. Sensitivity and specificity were evaluated, which demonstrated high sensitivity for both the singleplex and dual-target qRT-PCR assays with no cross-reaction with common bunyaviruses and tick-borne viruses. The TaqMan-based qRT-PCR methodology established in this study can be employed for epidemiological surveillance and pathogenesis studies of TAMV.

Keywords: Bunyaviruses; Real-time reverse transcription PCR; Tamdy virus; Tick-borne virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Orthobunyavirus*
Real-Time Polymerase Chain Reaction / methods
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcription
Sensitivity and Specificity
Thogotovirus*
Ticks*