Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead

Josie Chrenek; Rebecca Kirsch; Kali Scheck; Stephanie M Willerth

doi:10.1016/j.xpro.2022.101348

Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead

STAR Protoc. 2022 Apr 22;3(2):101348. doi: 10.1016/j.xpro.2022.101348. eCollection 2022 Jun 17.

Authors

Josie Chrenek¹, Rebecca Kirsch², Kali Scheck², Stephanie M Willerth^{1

2

3

4}

Affiliations

¹ Department of Biomedical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada.
² Department of Mechanical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada.
³ Division of Medical Sciences, University of Victoria, Victoria, BC V8W 2Y2, Canada.
⁴ School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.

Abstract

3D bioprinting-a type of additive manufacturing-can create 3D tissue constructs resembling in vivo tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences' fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be applied to printing 3D tissue models using a variety of cell lines and any chemically crosslinked bioink. These 3D-printed tissue models can be used for applications such as drug screening and disease modeling in vitro.

Keywords: Biotechnology and bioengineering; Material sciences; Neuroscience; Stem Cells; Tissue Engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bioprinting*
Nerve Tissue*
Printing, Three-Dimensional
Tissue Engineering / methods
Tissue Scaffolds