PATZ1 Induces Apoptosis through PUMA in Glioblastoma

Xiang Tao; Ge Zhang; Junhui Liu; Baowei Ji; Haitao Xu; Zhibiao Chen

doi:10.1155/2022/4953107

PATZ1 Induces Apoptosis through PUMA in Glioblastoma

J Oncol. 2022 Apr 25:2022:4953107. doi: 10.1155/2022/4953107. eCollection 2022.

Authors

Xiang Tao¹, Ge Zhang¹, Junhui Liu¹, Baowei Ji¹, Haitao Xu¹, Zhibiao Chen¹

Affiliation

¹ Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060 Hubei Province, China.

Abstract

Aim: This study was aimed at investigating the mechanism of PATZ1 inducing apoptosis through PUMA in glioblastoma. Overexpressed PATZ1 was transfected to explore its role in inducing apoptosis in glioblastoma cells.

Methods: The expression of protein was detected by western blotting assay. qRT-PCR assay was used to detect the expression of RNA. Confocal microscopy was used to analyze the correlation between PATZ1 and PUMA. TUNEL assay was used to detect the cell apoptosis. The ability of cell proliferation was detected by MTT assay and EDU assay. The effects of PATZ1 on cell apoptosis and tumor proliferation were observed in vivo by tumor xenograft mouse model.

Results: The results showed that low PATZ1 expression correlates with poor prognosis in glioblastoma patients. Overexpression of PATZ1 inhibits glioma cell proliferation and induces apoptosis by activating intrinsic apoptotic pathways. PATZ1 colocalizes intracellularly with PUMA inducing apoptosis through PUMA in glioblastoma.

Conclusion: PATZ1 plays a biological regulatory role in inducing apoptosis in glioblastoma, and this regulatory effect is related to PUMA, and the specific mechanism remains to be further explored.