Diversity in Phenotypes Associated With Host Persistence and Systemic Virulence in Streptococcus sanguinis Strains

Livia A Alves; Geovanny C Salvatierra; Victor A Freitas; José F Höfling; Débora C Bastos; Thaís L S Araujo; Renata O Mattos-Graner

doi:10.3389/fmicb.2022.875581

Diversity in Phenotypes Associated With Host Persistence and Systemic Virulence in Streptococcus sanguinis Strains

Front Microbiol. 2022 Apr 18:13:875581. doi: 10.3389/fmicb.2022.875581. eCollection 2022.

Authors

Livia A Alves¹, Geovanny C Salvatierra¹, Victor A Freitas¹, José F Höfling¹, Débora C Bastos^{2

3}, Thaís L S Araujo⁴, Renata O Mattos-Graner¹

Affiliations

¹ Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil.
² Department of Biosciences, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil.
³ São Leopoldo Mandic Medical School, Campinas, Brazil.
⁴ Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.

Abstract

Streptococcus sanguinis is a pioneer commensal species of dental biofilms, abundant in different oral sites and commonly associated with opportunist cardiovascular infections. In this study, we addressed intra-species functional diversity to better understand the S. sanguinis commensal and pathogenic lifestyles. Multiple phenotypes were screened in nine strains isolated from dental biofilms or from the bloodstream to identify conserved and strain-specific functions involved in biofilm formation and/or persistence in oral and cardiovascular tissues. Strain phenotypes of biofilm maturation were independent of biofilm initiation phenotypes, and significantly influenced by human saliva and by aggregation mediated by sucrose-derived exopolysaccharides (EPS). The production of H₂O₂ was conserved in most strains, and consistent with variations in extracellular DNA (eDNA) production observed in few strains. The diversity in complement C3b deposition correlated with the rates of opsonophagocytosis by human PMN and was influenced by culture medium and sucrose-derived EPS in a strain-specific fashion. Differences in C3b deposition correlated with strain binding to recognition proteins of the classical pathway, C1q and serum amyloid protein (SAP). Importantly, differences in strain invasiveness into primary human coronary artery endothelial cells (HCAEC) were significantly associated with C3b binding, and in a lesser extent, with binding to host glycoproteins (such as fibrinogen, plasminogen, fibronectin, and collagen). Thus, by identifying conserved and strain-specific phenotypes involved in host persistence and systemic virulence, this study indicates potential new functions involved in systemic virulence and highlights the need of including a wider panel of strains in molecular studies to understand S. sanguinis biology.

Keywords: Streptococcus sanguinis; biofilm; cardiovascular infections; complement system; eDNA; endothelial cells; exopolysaccharides.