Construction of the UDP-Glucose Biosynthetic Enzyme Gene Coexpression Plasmid for Prunasin Production in Escherichia coli

Methods Mol Biol. 2022:2469:19-28. doi: 10.1007/978-1-0716-2185-1_2.

Abstract

Microbial production of bioactive glucosides using uridine diphosphate glucosyltransferase (UGT) is an efficient glucoside production method. Here, we describe a detailed method for the construction of a UDP-glucose biosynthetic enzyme gene coexpression plasmid, that is, pCDF-PGP and the microbial production of prunasin from racemic mandelonitrile using Escherichia coli possessing UGT85A47 obtained from Japanese apricot. Furthermore, this constructed vector can find application in the production of various other glucosides that utilize other UGTs and aglycons.

Keywords: Cyanogenic glucoside; Escherichia coli; Japanese apricot; Microbial production; Prunasin; UDP-glucose; UDP-glucosyltransferase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli* / genetics
  • Glucose
  • Glucosides
  • Nitriles
  • Plasmids / genetics
  • Uridine Diphosphate
  • Uridine Diphosphate Glucose*

Substances

  • Glucosides
  • Nitriles
  • prunasin
  • Uridine Diphosphate
  • Glucose
  • Uridine Diphosphate Glucose