Characterization on structure and bioactivities of an exopolysaccharide from Lactobacillus curvatus SJTUF 62116

Abstract

This study aimed to investigate the chemical structure, physicochemical properties, antioxidant capacity, antibacterial ability and anti-biofilm formation activity of an exopolysaccharide (EPS) produced by Lactobacillus curvatus SJTUF 62116 from the fish Gymnocypris przewalskii. The purified EPS, denoted as EPS-1, was mainly composed of glucose and mannose at a relative molar ratio of 1:1.05 with molecular weight of 31.9 kDa. The chemical structure of EPS-1 was consisted of →2)-α-D-Manp-(1→, →4)-α-D-Manp-(1→, →3,6)-α-D-Manp-(1→, T-β-D-Glcp-(1→, →6)-β-D-Glcp-(1→, and →3)-β-D-Glcp-(1→ glycosidic bonds. A sheet-like structure of dried EPS-1 was determined by scanning electron microscope (SEM), whilst a peak-shaped structure of EPS-1 was observed by atomic force microscope (AFM). The degradation temperature of EPS-1 was determined as 300.21 °C using thermogravimetric analysis (TGA). Moreover, the antioxidant capacity of EPS-1 at a concentration of 5.0 mg/mL against DPPH and ABTS was 84.50% and 92.53%, respectively. Furthermore, EPS-1 exhibited acceptable bacteriostatic efficacy against S. Enteritidis, E. coli, and S.aureus with significant inhibition of S. Enteritidis biofilm formation.

Keywords: Anti-biofilm formation activity; Antibacterial activity; Antioxidation activity; Exopolysaccharide; Lactobacillus curvatus.