Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells

Petra den Hollander; Robiya Joseph; Suhas Vasaikar; Nick A Kuburich; Abhijeet P Deshmukh; Sendurai A Mani

doi:10.1007/978-1-0716-1979-7_38

Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells

Methods Mol Biol. 2022:2429:547-554. doi: 10.1007/978-1-0716-1979-7_38.

Authors

Petra den Hollander¹, Robiya Joseph¹, Suhas Vasaikar¹, Nick A Kuburich¹, Abhijeet P Deshmukh¹, Sendurai A Mani²

Affiliations

¹ Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
² Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. smani@mdanderson.org.

PMID: 35507188
DOI: 10.1007/978-1-0716-1979-7_38

Abstract

Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. Calculating the frequency of tumor-initiating cells is important in the assessment of the number of CSCs present in a cell population. In this chapter, we present a protocol developed for quantification of CSCs from breast cancer tumors that can be adapted to CSCs from other types of tumors.

Keywords: Cancer stem cells; In vivo; Limiting dilution tumor initiation; Tumor-initiating cell frequency.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms* / pathology
Cell Transformation, Neoplastic / metabolism
Female
Humans
Neoplastic Stem Cells* / metabolism

Abstract

Publication types

MeSH terms

Grants and funding