Reprogramming Mouse Oviduct Epithelial Cells Using In Vivo Electroporation and CRISPR/Cas9-Mediated Genetic Manipulation

Matthew J Ford; Yojiro Yamanaka

doi:10.1007/978-1-0716-1979-7_24

Reprogramming Mouse Oviduct Epithelial Cells Using In Vivo Electroporation and CRISPR/Cas9-Mediated Genetic Manipulation

Methods Mol Biol. 2022:2429:367-377. doi: 10.1007/978-1-0716-1979-7_24.

Authors

Matthew J Ford¹, Yojiro Yamanaka²

Affiliations

¹ Department of Human Genetics, Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, Quebec, Canada.
² Department of Human Genetics, Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, Quebec, Canada. yojiro.yamanaka@mcgill.ca.

PMID: 35507174
DOI: 10.1007/978-1-0716-1979-7_24

Abstract

Advances in gene editing tools such as CRISPR/Cas9 have made precise in vivo gene editing possible, opening up avenues of research into somatic cell reprograming to study adult stem cells, homeostasis, and malignant transformation. Here we describe a method for CRISPR/Cas9 mediated in vivo gene editing, in combination with Cre-based lineage tracing via electroporation in the mouse oviduct. This method facilitates the delivery of multiple plasmids into oviduct epithelial cells, sufficient for studying homeostasis and generation of high-grade serous ovarian cancer (HGSOC) models.

Keywords: CRISPR; Fallopian tube; HGSOC; Homeostasis; In vivo gene editing; Ovarian cancer; Oviduct.

MeSH terms

Animals
CRISPR-Cas Systems* / genetics
Electroporation / methods
Epithelial Cells
Fallopian Tubes
Female
Gene Editing* / methods
Humans
Mice