Effectiveness of agricultural waste in the enhancement of biological denitrification of aquaculture wastewater

Shuwei Gao; Wangbao Gong; Kai Zhang; Zhifei Li; Guangjun Wang; Ermeng Yu; Yun Xia; Jingjing Tian; Hongyan Li; Jun Xie

doi:10.7717/peerj.13339

Effectiveness of agricultural waste in the enhancement of biological denitrification of aquaculture wastewater

PeerJ. 2022 Apr 28:10:e13339. doi: 10.7717/peerj.13339. eCollection 2022.

Authors

Shuwei Gao^{1

2

3}, Wangbao Gong^{1

2}, Kai Zhang^{1

2}, Zhifei Li^{1

2}, Guangjun Wang^{1

2}, Ermeng Yu^{1

2}, Yun Xia^{1

2}, Jingjing Tian^{1

2}, Hongyan Li^{1

2}, Jun Xie^{1

2}

Affiliations

¹ Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, China.
² Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, China.
³ National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.

Abstract

Nitrogen pollution in aquaculture wastewater can pose a significant health and environmental risk if not removed before wastewater is discharged. Biological denitrification uses external carbon sources to remove nitrogen from wastewater; however, these carbon sources are often expensive and require significant energy. In this study, we investigated how six types of agricultural waste can be used as solid carbon sources in biological denitrification. Banana stalk (BS), loofah sponge (LS), sorghum stalk (SS), sweet potato stalk (SPS), watermelon skins (WS) and wheat husk (WH) were studied to determine their capacity to release carbon and improve denitrification efficiency. The results of batch experiments showed that all six agricultural wastes had excellent carbon release capacities, with cumulative chemical oxygen demands of 37.74-535.68 mg/g. During the 168-h reaction, the carbon release process followed the second-order kinetic equation and Ritger-Peppas equation, while carbon release occurred via diffusion. The kinetic equation fitting, scanning electron microscopy, and Fourier transform infrared spectroscopy results showed that LS had the lowest c_m and the maximum t_1/2 values and only suffered a moderate degree of hydrolysis. It also had the lowest pollutant release rate and cumulative chemical oxygen demand, as well as the most efficient removal of total phosphorous (TP) and total nitrogen (TN). Therefore, we concluded that LS has the lowest potential risk of excess carbon release and capacity for long-lasting and stable carbon release. The WS leachate had the highest TN contents, while the SPS leachate had the highest TP content. In the 181-h denitrification reaction, all six agricultural wastes completely removed nitrate and nitrite; however, SS had the highest denitrification rate, followed by LS, WH, BS, SPS, and WS (2.16, 1.35, 1.35, 1.34, 1.34, and 1.01 mg/(L·h), respectively). The denitrification process followed a zero-order and first-order kinetic equation. These results provide theoretical guidance for effectively selecting agricultural waste as a solid carbon source and improving the denitrification efficiency of aquaculture wastewater treatment.

Keywords: Agricultural wastes; Aquaculture wastewater; Denitrification; Kinetic; Solid carbon sources.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aquaculture
Bioreactors
Carbon / chemistry
Denitrification
Musa*
Nitrogen / chemistry
Phosphorus
Wastewater*

Substances

Wastewater
Carbon
Nitrogen
Phosphorus

Grants and funding

This work was supported by the National Key R&D Program of China (No. 2019YFD0900302) and the Special Fund for Fishery Economic Development of Guangdong Province, China (No. 2019B13). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.