Simultaneous quantification of fourteen characteristic active compounds in Eucommia ulmoides Oliver and its tea product by ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS)

Xin-Yue Li; Yu-Jie Fu; Yue-Feng Fu; Wei Wei; Cheng Xu; Xiao-Han Yuan; Cheng-Bo Gu

doi:10.1016/j.foodchem.2022.133106

Simultaneous quantification of fourteen characteristic active compounds in Eucommia ulmoides Oliver and its tea product by ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS)

Food Chem. 2022 Sep 30:389:133106. doi: 10.1016/j.foodchem.2022.133106. Epub 2022 Apr 29.

Authors

Xin-Yue Li¹, Yu-Jie Fu², Yue-Feng Fu¹, Wei Wei¹, Cheng Xu¹, Xiao-Han Yuan³, Cheng-Bo Gu⁴

Affiliations

¹ Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, PR China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Harbin 150040, PR China.
² Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Forestry, Beijing Forestry University, Beijing 100083, PR China.
³ Life Science and Biotechnique Research Center, Northeast Agricultural University, Harbin 150030, PR China.
⁴ Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, PR China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Harbin 150040, PR China. Electronic address: dilisatis@163.com.

PMID: 35504080
DOI: 10.1016/j.foodchem.2022.133106

Abstract

Various kinds of bioactive compounds contribute to versatile health-promoting properties of Eucommia ulmoides Oliver (E. ulmoides). In present study, we developed a UPLC-QqQ-MS/MS method for simultaneous quantification of fourteen characteristic active compounds, including 3 lignans, 4 iridoids, 3 flavonoids and 4 phenolics in E. ulmoides and its tea product for the first time. The running time of the method is 6.5 min. It has good linearity, sensitivity, precision, accuracy, and stability. Using this high-throughput method, the distributions of fourteen characteristic active compounds in E. ulmoides and its tea product were clarified. Also, it was found that E. ulmoides tea exhibited superiority in contents of chlorogenic acid as compared with natural resources. Overall, the study provided a rapid, reliable, and efficient analysis method, which could be applied for the quality evaluation of E. ulmoides natural resources and their relative products in the field of food and medicine.

Keywords: Eucommia ulmoides Oliver; Eucommia ulmoides Oliver tea; Multiple reaction monitoring; Quality control; UPLC-QqQ–MS/MS.

MeSH terms

Chlorogenic Acid
Chromatography, High Pressure Liquid / methods
Eucommiaceae* / chemistry
Tandem Mass Spectrometry / methods
Tea

Substances

Tea
Chlorogenic Acid