Advanced tools and methods for single-cell surgery

Adnan Shakoor; Wendi Gao; Libo Zhao; Zhuangde Jiang; Dong Sun

doi:10.1038/s41378-022-00376-0

Advanced tools and methods for single-cell surgery

Microsyst Nanoeng. 2022 Apr 29:8:47. doi: 10.1038/s41378-022-00376-0. eCollection 2022.

Authors

Adnan Shakoor^#¹, Wendi Gao^#², Libo Zhao², Zhuangde Jiang², Dong Sun^{1

2}

Affiliations

¹ Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China.
² State Key Laboratory for Manufacturing Systems Engineering, International Joint Laboratory for Micro/Nano Manufacturing and Measurement Technologies, The School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an, China.

^# Contributed equally.

Abstract

Highly precise micromanipulation tools that can manipulate and interrogate cell organelles and components must be developed to support the rapid development of new cell-based medical therapies, thereby facilitating in-depth understanding of cell dynamics, cell component functions, and disease mechanisms. This paper presents a literature review on micro/nanomanipulation tools and their control methods for single-cell surgery. Micromanipulation methods specifically based on laser, microneedle, and untethered micro/nanotools are presented in detail. The limitations of these techniques are also discussed. The biological significance and clinical applications of single-cell surgery are also addressed in this paper.

Keywords: Engineering; Nanobiotechnology.

Publication types

Review