Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model

Jian-Bo Song; Rui-Ke Huang; Miao-Jie Guo; Qian Zhou; Rui Guo; Shu-Yuan Zhang; Jing-Wen Yao; Ya-Ni Bai; Xuan Huang

doi:10.7717/peerj.13293

Lipids associated with plant-bacteria interaction identified using a metabolomics approach in an Arabidopsis thaliana model

PeerJ. 2022 Apr 27:10:e13293. doi: 10.7717/peerj.13293. eCollection 2022.

Authors

Jian-Bo Song^{1

2}, Rui-Ke Huang^{1

2}, Miao-Jie Guo^{1

2}, Qian Zhou³, Rui Guo^{1

2}, Shu-Yuan Zhang^{1

2}, Jing-Wen Yao^{1

2}, Ya-Ni Bai^{1

2}, Xuan Huang^{1

2}

Affiliations

¹ College of Life Sciences, Northwest University, Shaanxi, Xi'an, China.
² Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Provincial Key Laboratory of Biotechnology of Shaanxi, Shaanxi, Xi'an, China.
³ Shanghai Omicsspace Biotechnology Co.Ltd., Shanghai, Shanghai, China.

Abstract

Background: Systemic acquired resistance (SAR) protects plants against a wide variety of pathogens. In recent decades, numerous studies have focused on the induction of SAR, but its molecular mechanisms remain largely unknown.

Methods: We used a metabolomics approach based on ultra-high-performance liquid chromatographic (UPLC) and mass spectrometric (MS) techniques to identify SAR-related lipid metabolites in an Arabidopsis thaliana model. Multiple statistical analyses were used to identify the differentially regulated metabolites.

Results: Numerous lipids were implicated as potential factors in both plant basal resistance and SAR; these include species of phosphatidic acid (PA), monogalactosyldiacylglycerol (MGDG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG).

Conclusions: Our findings indicate that lipids accumulated in both local and systemic leaves, while other lipids only accumulated in local leaves or in systemic leaves. PA (16:0_18:2), PE (34:5) and PE (16:0_18:2) had higher levels in both local leaves inoculated with Psm ES4326 or Psm avrRpm1 and systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. PC (32:5) had high levels in leaves inoculated with Psm ES4326. Other differentially regulated metabolites, including PA (18:2_18:2), PA (16:0_18:3), PA (18:3_18:2), PE (16:0_18:3), PE (16:1_16:1), PE (34:4) and TGs showed higher levels in systemic leaves of the plants locally infected with Psm avrRpm1 or Psm ES4326. These findings will help direct future studies on the molecular mechanisms of SAR.

Keywords: Lipids; Monogalactosyldiacylglycerol; Phosphatidic acid; Phosphatidylethanolamine; Systemic acquired resistance; Triacylglycerol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis Proteins* / metabolism
Arabidopsis* / metabolism
Bacteria / metabolism
Metabolomics
Pseudomonas syringae / metabolism

Substances

Arabidopsis Proteins

Grants and funding

This study was supported by grants from the National Natural Science Foundation of China (31300223), the Major Project of Basic Research Program of Natural Sciences of Shaanxi Province (2021JZ-41), Natural Science Foundation of Shaanxi Province (2016JM3001), Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, First-class University and Academic programs of Northwest University, Northwest University Graduate Innovation and Creativity Funds (YZZ17152), and National Training Programs of Innovation and Entrepreneurship for Undergraduate (201910697021). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.