Titanium platelet-rich fibrin (T-PRF) as high-capacity doxycycline delivery system

Esra Ercan; Selin S Suner; Coskun Silan; Selehattin Yilmaz; Duygu Siddikoglu; Nurettin Sahiner; Mustafa Tunali

doi:10.1007/s00784-022-04510-0

Titanium platelet-rich fibrin (T-PRF) as high-capacity doxycycline delivery system

Clin Oral Investig. 2022 Aug;26(8):5429-5438. doi: 10.1007/s00784-022-04510-0. Epub 2022 May 3.

Authors

Esra Ercan¹, Selin S Suner², Coskun Silan³, Selehattin Yilmaz², Duygu Siddikoglu⁴, Nurettin Sahiner^{2

5

6}, Mustafa Tunali⁷

Affiliations

¹ Department of Periodontology, Faculty of Dentistry, Canakkale Onsekiz Mart University, 17110, Canakkale, Turkey. esraercan82@gmail.com.
² Department of Chemistry, Faculty of Sciences & Arts, and Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale, 17100, Turkey.
³ Department of Pharmacology, Faculty of Medicine, Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale, 17100, Turkey.
⁴ Department of Biostatistics, Faculty of Medicine, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100, Canakkale, Turkey.
⁵ Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, FL, 33620, USA.
⁶ Department of Chemical & Biomedical Engineering, and Materials Science and Engineering Program, University of South Florida, Tampa, FL, 33620, USA.
⁷ Department of Periodontology, Faculty of Dentistry, Canakkale Onsekiz Mart University, 17110, Canakkale, Turkey.

PMID: 35501503
DOI: 10.1007/s00784-022-04510-0

Abstract

Objectives: Titanium platelet-rich fibrin (T-PRF), a second-generation autogenous blood concentrate with tough and thick fibrin meshwork activated by a titanium tube, was used as a drug carrier for doxycycline (Doxy) by injection. The objective of this study is to evaluate the loading capacity of T-PRF, release kinetics of doxycycline-loaded T-PRF, and its antibacterial effects against S. aureus and P. aeruginosa.

Materials and methods: The T-PRF and collagen were loaded with Doxy as T-PRF/Doxy and Collagen/Doxy, and their release and antibacterial activities against S. aureus and P. aeruginosa were investigated. Chemical characterization and morphological analysis were performed.

Results: In comparison with collagen, approximately sevenfold more Doxy, 281 mg/g, was loaded into T-PRF. It was found that 25% of the loaded Doxy was released from T-PRF compared to only 12% from collagen within 72 h. The largest inhibition zone diameter (IZD) was observed for T-PRF/Dox with 32 ± 6 mm and 37 ± 5 mm for P. aereginosa and S. aureus, respectively. However, only 10 ± 5 mm and 10 ± 6 mm IZD were observed for bare T-PRF, and no inhibition zone was observed for the Collagen/Doxy group. A dense fibrin structure was visualized on SEM images of the T-PRF/Doxy group compared to the T-PRF group.

Conclusions: T-PRF has higher Doxy loading capacity and long-acting antibacterial effects compared to collagen. T-PRF was shown to have potential autogenous long-term drug-carrying capability for doxycycline. Also, the potential fibrinophilic properties of Doxy were observed to strengthen the structure of T-PRF.

Clinical relevance: T-PRF is an autogenous drug career with high loading capacity and extended antibacterial effects for doxycycline. Doxycycline molecules can be visible on T-PRF fibers. This study suggests that T-PRF/Dox could be used as a proper antibiotic delivery device in the treatments of periodontitis and peri-implantitis.

Keywords: Doxycycline; Drug delivery; P. aeruginosa; S. aureus; T-PRF; Titanium platelet–rich fibrin.

MeSH terms

Anti-Bacterial Agents / administration & dosage
Doxycycline* / administration & dosage
Fibrin
Platelet-Rich Fibrin*
Staphylococcus aureus / drug effects
Titanium / chemistry

Substances

Anti-Bacterial Agents
Fibrin
Titanium
Doxycycline