Hydrochar amendments stimulate soil nitrous oxide emission by increasing production of hydroxyl radicals and shifting nitrogen functional genes in the short term: A culture experiment

Abstract

The application of waste biomass-derived hydrochar to soil may cause extremely intensive nitrous oxide (N₂O) fluxes that can challenge our current mechanistic understanding of the global nitrogen cycle in the biosphere. In this study, two waste biomasses were used to prepare cyanobacterial biomas-derived hydrochar (CHC) and wheat straw-derived hydrochar (SHC) for short-term incubation experiments to identify their effects and mechanisms of waste biomass-derived hydrochar on soil N₂O efflux, with time-series samples collected for N₂O efflux and soil analysis. The results showed that CHC and SHC caused short-term bursts of N₂O effluxes without nitrogen inputs. Moreover, the enrichment of exogenous organics and nutrients at the hydrochar-soil interface was identified as the key factor for enhancing N₂O fluxes, which stimulated microbial nitrification (i.e., increased gene copy number of ammonia oxidizing bacteria) and denitrification (i.e., increased gene copy number of nitrate and N₂O reducing bacteria) processes. The concentrations of Fe (II) and hydroxyl radicals (HO^•) were 6.49 and 5.63 times higher, respectively, in the hydrochar layer of CHC than SHC amendment. Furthermore, structural equation models demonstrated that HO^•, as well as soil microbiomes, played an important role in driving N₂O fluxes. Together, our findings provide a deeper insight into the assessment and prognosis of the short-term environmental risk arising from agricultural waste management in integrated agriculture. Further studies under practical field application conditions are warranted to verify the findings.

Keywords: Functional genes; Hydrochar; Hydroxyl radicals; Nitrous oxide; Soil; Waste biomass.