Spectrophotometric Methods for Determination of Dopamine Hydrochloride in Bulk and in Injectable Forms

Najat O A Al-Salahi; Elham Y Hashem; Doaa A Abdel-Kader

doi:10.1016/j.saa.2022.121278

Spectrophotometric Methods for Determination of Dopamine Hydrochloride in Bulk and in Injectable Forms

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 5:278:121278. doi: 10.1016/j.saa.2022.121278. Epub 2022 Apr 20.

Authors

Najat O A Al-Salahi¹, Elham Y Hashem², Doaa A Abdel-Kader³

Affiliations

¹ Chemistry Department, Faculty of Science, Abyan University, Yemen.
² Chemistry Department, Faculty of Science, Assiut University, 71516, Egypt. Electronic address: elhamhashem@science.aun.edu.eg.
³ Chemistry Department, Faculty of Science, Assiut University, 71516, Egypt.

PMID: 35500351
DOI: 10.1016/j.saa.2022.121278

Abstract

Two highly simple, economical, accurate and sensitive spectrophotometric methods for determination of dopamine hydrochloride (DAH) in either pure form and pharmaceutical formulations are described. The first method is based on determination of (DAH) spectrophotometrically at maximum absorbance 280 nm (method A). The second one is based on reduction of alkaline KMnO₄ by (DAH) leading to the formation of green manganate species which are measured at 610 nm (method B). Under optimized conditions, the calibration graphs were linear in the range of 3.793 - 45.513, 0.190 - 4.362 μg.mL^-1 of (DAH) for methods A and B respectively. The developed methods were successfully applied for the determination of dopamine hydrochloride in pharmaceutical samples.

Keywords: Dopamine hydrochloride; Oxidation; Pharmaceutical Formulations; Potassium permanganate or KMnO(4); UV spectrophotometry.

Published by Elsevier B.V.

MeSH terms

Calibration
Dopamine / analysis*
Manganese / chemistry
Pharmaceutical Preparations / classification
Spectrophotometry / methods*

Substances

Pharmaceutical Preparations
Manganese
Dopamine