Effective Detection of Phenylalanine Using Pyridine Based Sensor

Vijayakumar Sathya; Venkatesan Srinivasadesikan; Shyi-Long Lee; Vediappen Padmini

doi:10.1007/s10895-022-02944-z

Effective Detection of Phenylalanine Using Pyridine Based Sensor

J Fluoresc. 2022 Jul;32(4):1481-1488. doi: 10.1007/s10895-022-02944-z. Epub 2022 May 2.

Authors

Vijayakumar Sathya¹, Venkatesan Srinivasadesikan², Shyi-Long Lee³, Vediappen Padmini⁴

Affiliations

¹ Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamilnadu, India.
² Division of Chemistry, Department of Sciences and Humanities, Vignans Foundation for Science, Technology and Research, Vadlamudi, Guntur, 522 213, Andhra Pradesh, India.
³ Department of Chemistry and Biochemistry, National Chung-Cheng University, Chia-Yi, 621, Taiwan.
⁴ Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamilnadu, India. padmini.chem@mkuniversity.org.

PMID: 35499662
DOI: 10.1007/s10895-022-02944-z

Abstract

Pyridine based organic molecule as probe has been synthesized for the detection of phenylalanine (PA) biomarker. The synthesized probe is characterized by ¹H and ¹³C NMR and mass spectroscopic studies. The photophysical properties for the probe has recorded by colorimetric and fluorimetric techniques. The quenching has been observed between the probe and PA through ICT (Intermolecular Charge Transfer Mechanism). Under optimized conditions, the probe detects PA selectively in the presence of other biologically important biomolecules. The practical application for PA has been successfully applied in human blood serum and urine.

Keywords: Fluorescence; Intermolecular Charge Transfer Mechanism (ICT); Phenylalanine; Phenylketonuria; Pyridine derivatives; Sensor.

MeSH terms

Colorimetry / methods
Fluorescent Dyes* / chemistry
Humans
Phenylalanine*
Pyridines

Substances

Fluorescent Dyes
Pyridines
Phenylalanine