Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice

Hualing Xie; Jingyu Fang; Mohamed A Farag; Zhenhao Li; Peilong Sun; Ping Shao

doi:10.1016/j.fochx.2022.100235

Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice

Food Chem X. 2022 Jan 29:13:100235. doi: 10.1016/j.fochx.2022.100235. eCollection 2022 Mar 30.

Authors

Hualing Xie¹, Jingyu Fang¹, Mohamed A Farag^{2

3}, Zhenhao Li⁴, Peilong Sun¹, Ping Shao¹

Affiliations

¹ College of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, PR China.
² Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr El Aini St. P.B, Cairo, Egypt.
³ Department of Chemistry, School of Science & Engineering, The American University in Cairo, New Cairo 11835, Egypt.
⁴ Zhejiang ShouXianGu Botanical Drug Institute Co. Ltd, Zhejiang, Hangzhou 321200, China.

Abstract

In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases.

Keywords: ALT, alanine aminotransferase; AST, aspartate aminotransferase; Cyclophosphamide; Cys, cyclophosphamide monohydrate; DOLP, Dendrobium officinale leaf polysaccharides; Dendrobium officinale; Gut microbiota; IgA, immunoglobulin A; Immune system; LH, levamisole hydrochloride; Liver protection; OTUs, operational taxonomic units; Polysaccharide fermentation; SCFA, short chain fatty acid.