Gracilaria lemaneiformis polysaccharides alleviate colitis by modulating the gut microbiota and intestinal barrier in mice

Si-Yuan Lu; Yang Liu; Shijie Tang; Wancong Zhang; Qiuyong Yu; Changqi Shi; Kit-Leong Cheong

doi:10.1016/j.fochx.2021.100197

Gracilaria lemaneiformis polysaccharides alleviate colitis by modulating the gut microbiota and intestinal barrier in mice

Food Chem X. 2021 Dec 22:13:100197. doi: 10.1016/j.fochx.2021.100197. eCollection 2022 Mar 30.

Authors

Si-Yuan Lu¹, Yang Liu¹, Shijie Tang^{2

3}, Wancong Zhang^{2

3}, Qiuyong Yu⁴, Changqi Shi⁴, Kit-Leong Cheong¹

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Biotechnology, Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, China.
² Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
³ Plastic Surgery Institute of Shantou University Medical College, Shantou, Guangdong, China.
⁴ Maynntetra (Shantou) Bio-technology Co., Ltd., Shantou, Guangdong, China.

Abstract

Gracilaria lemaneiformis polysaccharide (GLP) has varieties of antioxidation, however, the therapeutic effects of GLP on ulcerative colitis (UC) and the potential mechanisms involved are still incomplete. In the study, the analysis of the ζ-potential, thermal, and morphology properties demonstrated that GLP was a negatively charged polymer, and had great thermostability and irregular network. Moreover, the GLP treatment has the effects of reducing the severity of colitis caused by dextran sulfate sodium by alleviating the colon damage of mice, and increasing the amount of short-chain fatty acids in the intestines, alleviating histopathological inflammation. The sequencing results and α-diversity analysis showed that GLP could improve biodiversity, restore the abundance of Bacteroidetes, and decrease the proportion of Firmicutes. The level of CCL-25 and CCR-9 were inhibited, CD40 and TGF-β1 were increased. In summary, GLP has potentiality to be utilized as a hopeful functional food to the UC patients.

Keywords: Colitis; Gracilaria lemaneiformis; Gut microbiota; In vivo; Polysaccharide.