Biotechnological formation of dairy flavor inducing δ-lactones from vegetable oil

H Zia; U Von Ah; Y H Meng; R Schmidt; J Kerler; P Fuchsmann

doi:10.1016/j.fochx.2022.100220

Biotechnological formation of dairy flavor inducing δ-lactones from vegetable oil

Food Chem X. 2022 Jan 19:13:100220. doi: 10.1016/j.fochx.2022.100220. eCollection 2022 Mar 30.

Authors

H Zia¹, U Von Ah¹, Y H Meng¹, R Schmidt¹, J Kerler², P Fuchsmann¹

Affiliations

¹ Agroscope, Schwarzenburgstrasse 161, 3003 Bern, Switzerland.
² Nestlé Technology Centre Dairy, Nestlé Strasse 3, 3510 Konolfingen, Switzerland.

Abstract

Agroscope Culture Collection was screened to identify bacterial strains effective in production of dairy flavor inducing lactones using grapeseed oil as a substrate. Lentilactobacillus parafarraginis FAM-1079, Lactococcus lactis subsp. lactis FAM-17918, and L. lactis subsp. lactis biovar diacetylactis FAM-22003 showed the most efficient formation of targeted δ-lactones. The application of sublethal heat stress significantly increased target lactone production. The most profound improvement was for L. lactis subsp. lactis biovar diacetylactis where δ-octadecalactone generation was improved by factor of 9. The pre-fermentation step as well as growth phase in which bacteria are harvested did not have a significant impact on lactones yield. The lactone production process from vegetable oil developed in this study offers a new way of developing a natural flavor ingredient for incorporation into plant-based products.

Keywords: ACC, Agroscope Culture Collection; Aroma-active lactones; ESP, Early stationary phase; Fermentation; Flavor generation; GC-O, Gas-chromatography–olfactometry; HFA, Hydroxy Fatty acid; HS, Head space; Heat stress; LAB, Lactic acid bacteria; LOQ, Limit of quantitation; Lactic acid bacteria; MEP, Mid-exponential phase; OD, Optical density; QF, Qualifier ion; QT, Quantifier ion; RI, Retention index; SPE, Solid phase extraction; UFA, Unsaturated fatty acid; Vegetable oil; δ-C10, δ-decalactone; δ-C12, δ-dodecalactone; δ-C14, δ-tetradecalactone; δ-C18, δ-octadecalactone.