Circulating FGF21 and GDF15 as Biomarkers for Screening, Diagnosis, and Severity Assessment of Primary Mitochondrial Disorders in Children

Yi Li; Shengrui Li; Yinfeng Qiu; Maobin Zhou; Min Chen; Yue Hu; Siqi Hong; Li Jiang; Yi Guo

doi:10.3389/fped.2022.851534

Circulating FGF21 and GDF15 as Biomarkers for Screening, Diagnosis, and Severity Assessment of Primary Mitochondrial Disorders in Children

Front Pediatr. 2022 Apr 14:10:851534. doi: 10.3389/fped.2022.851534. eCollection 2022.

Authors

Yi Li^{1

2

3

4}, Shengrui Li¹, Yinfeng Qiu¹, Maobin Zhou¹, Min Chen^{1

2

3

4}, Yue Hu^{1

2

3

4}, Siqi Hong^{1

2

3

4}, Li Jiang^{1

2

3

4}, Yi Guo^{1

2

3

4}

Affiliations

¹ Department of Neurology, Children's Hospital of Chongqing Medical University, Chongqing, China.
² National Clinical Research Center for Child Health and Disorders, Chongqing, China.
³ Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China.
⁴ Chongqing Key Laboratory of Pediatrics, Chongqing, China.

Abstract

Background: Primary mitochondrial disorders (PMDs) are a diagnostic challenge for paediatricians, and identification of reliable and easily measurable biomarkers has become a high priority. This study aimed to investigate the role of serum fibroblast growth factor 21 (FGF21) and growth differentiation factor 15 (GDF15) in children with PMDs.

Methods: We analysed serum FGF21 and GDF15 concentrations by enzyme-linked immunosorbent assay (ELISA) in children with PMDs, patients with non-mitochondrial neuromuscular disorders (NMDs), and aged-matched healthy children, and compared them with serum lactate and ratio of lactate and pyruvate (L/P). We also evaluated correlations between these biomarkers and the phenotype, genotype, and severity of PMDs.

Results: The median serum GDF15 and FGF21 concentrations were significantly elevated in fifty-one patients with PMDs (919.46 pg/ml and 281.3 pg/ml) compared with those of thirty patients with NMDs (294.86 pg/ml and 140.51 pg/ml, both P < 0.05) and fifty healthy controls (221.21 pg/ml and 85.02 pg/ml, both P < 0.05). The area under the curve of GDF15 for the diagnosis of PMDs was 0.891, which was higher than that of the other biomarkers, including FGF21 (0.814), lactate (0.863) and L/P ratio (0.671). Calculated by the maximum Youden index, the critical value of GDF15 was 606.369 pg/ml, and corresponding sensitivity and specificity were 74.5and 100%. In the PMD group, FGF21 was significantly correlated with International Paediatric Mitochondrial Disease Scale (IPMDS) score. The levels of GDF15 and FGF21 were positively correlated with age, critical illness condition, and multisystem involvement but were not correlated with syndromic/non-syndromic PMDs, different mitochondrial syndromes, nuclear DNA/mitochondrial DNA pathogenic variants, gene functions, or different organ/system involvement.

Conclusion: Regardless of clinical phenotype and genotype, circulating GDF15 and FGF21 are reliable biomarkers for children with PMDs. GDF15 can serve as a screening biomarker for diagnosis, and FGF21 can serve as a severity biomarker for monitoring.

Keywords: FGF21; GDF15; biomarkers; childhood; primary mitochondrial disorders.