Comprehensive Analysis of Cytochrome P450 Monooxygenases Reveals Insight Into Their Role in Partial Resistance Against Phytophthora sojae in Soybean

Praveen Khatri; Owen Wally; Istvan Rajcan; Sangeeta Dhaubhadel

doi:10.3389/fpls.2022.862314

Comprehensive Analysis of Cytochrome P450 Monooxygenases Reveals Insight Into Their Role in Partial Resistance Against Phytophthora sojae in Soybean

Front Plant Sci. 2022 Apr 14:13:862314. doi: 10.3389/fpls.2022.862314. eCollection 2022.

Authors

Praveen Khatri^{1

2}, Owen Wally³, Istvan Rajcan⁴, Sangeeta Dhaubhadel^{1

2}

Affiliations

¹ London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
² Department of Biology, University of Western Ontario, London, ON, Canada.
³ Harrow Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
⁴ Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada.

Abstract

Cytochrome P450 monooxygenases (P450) participate in the catalytic conversion of biological compounds in a plethora of metabolic pathways, such as the biosynthesis of alkaloids, terpenoids, phenylpropanoids, and hormones in plants. Plants utilize these metabolites for growth and defense against biotic and abiotic stress. In this study, we identified 346 P450 (GmP450) enzymes encoded by 317 genes in soybean where 26 GmP450 genes produced splice variants. The genome-wide comparison of both A-type and non-A-type GmP450s for their motifs composition, gene structure, tissue-specific expression, and their chromosomal distribution were determined. Even though conserved P450 signature motifs were found in all GmP450 families, larger variation within a specific motif was observed in the non-A-type GmP450s as compared with the A-type. Here, we report that the length of variable region between two conserved motifs is exact in the members of the same family in majority of the A-type GmP450. Analyses of the transcriptomic datasets from soybean-Phytophthora sojae interaction studies, quantitative trait loci (QTL) associated with P. sojae resistance, and co-expression analysis identified some GmP450s that may be, in part, play an important role in partial resistance against P. sojae. The findings of our CYPome study provides novel insights into the functions of GmP450s and their involvements in metabolic pathways in soybean. Further experiments will elucidate their roles in general and legume-specific function.

Keywords: Phytophthora sojae; QTL; cytochrome P450 monooxygenases; phytoalexins; soybean.

Copyright © 2022 Istvan Rajcan, and Her Majesty the Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada for the contribution of Praveen Khatri, Owen Wally, and Sangeeta Dhaubhadel.