Preparation and Characterization of Calcium-Incorporated Rosa roxburghii Tratt and Its Efficacy on Bone Mineral Density in Rats

Yanfang Yan; Zhongsheng Luo; Leilei He; Fuxiao Wei; Ming Gao; Xiaofang Cui; Juan Yang; Yichun Sun; Lilang Li; Lishou Yang; Tingfei Deng; Xiong Pan; Mei Peng; Yunfei Tan; Zhengbin An; Liangqun Li; Xiaosheng Yang

doi:10.1155/2022/5122396

Preparation and Characterization of Calcium-Incorporated Rosa roxburghii Tratt and Its Efficacy on Bone Mineral Density in Rats

Evid Based Complement Alternat Med. 2022 Apr 20:2022:5122396. doi: 10.1155/2022/5122396. eCollection 2022.

Authors

Yanfang Yan^{1

2}, Zhongsheng Luo^{1

2}, Leilei He^{1

2}, Fuxiao Wei^{1

2}, Ming Gao^{1

2}, Xiaofang Cui³, Juan Yang^{1

2}, Yichun Sun⁴, Lilang Li^{1

2}, Lishou Yang^{1

2}, Tingfei Deng^{1

2}, Xiong Pan^{1

2}, Mei Peng^{1

2}, Yunfei Tan^{1

2}, Zhengbin An^{1

2}, Liangqun Li^{1

2}, Xiaosheng Yang^{1

2}

Affiliations

¹ State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China.
² The Key Laboratory of Chemistry for Natural Products of Guizhou Province, Chinese Academy of Sciences, Guiyang 550014, China.
³ Guizhou Cancer Hospital, Guiyang 550008, China.
⁴ Sinopharm Group Tongjitang (Guizhou) Pharmaceutical Co., Ltd., Guiyang 550009, China.

Abstract

The deficiency of traditional calcium preparation will gradually be replaced by the new type of calcium preparation. Rosa roxburghii fruit (R. roxburghii) is popular for its rich nutrients and functional ingredients. The fermentation broth of R. roxburghii, involving amino acids, flavonoids, triterpenes, polysaccharides, and other compounds, is favorable for calcium chelation. Thus, this study fabricated calcium-incorporated R. roxburghii (FECa) and further illustrated its efficacy on bone mineral density (BMD) in rats. The calcium holding capacity of FECa was identified and confirmed using AAS. Ion complexation of FECa was characterized using ¹H-NMR, UV, SEM and EDS, and FTIR. The calcium contents of femurs were increased by 36%, and the bone trabeculae of femurs were significantly increased. Net calcium balance was enhanced to further improve BMD by oral administration of FECa. The above results indicate that FECa can be a potential and efficient calcium supplementation agent.