Identification of blood-activating components from Xueshuan Xinmaining Tablet based on the spectrum-effect relationship and network pharmacology analysis

Jing Tan; Junli Liu; Han Wang; Ying Zhang; Hongqiang Lin; Zhongyao Wang; Hanrui Si; Yutong Zhang; Jinping Liu; Pingya Li; Kai Sun

doi:10.1039/c9ra09623j

Identification of blood-activating components from Xueshuan Xinmaining Tablet based on the spectrum-effect relationship and network pharmacology analysis

RSC Adv. 2020 Mar 10;10(16):9587-9600. doi: 10.1039/c9ra09623j. eCollection 2020 Mar 2.

Authors

Jing Tan¹, Junli Liu¹, Han Wang¹, Ying Zhang^{1

2}, Hongqiang Lin¹, Zhongyao Wang¹, Hanrui Si¹, Yutong Zhang¹, Jinping Liu^{1

3}, Pingya Li^{1

3}, Kai Sun¹

Affiliations

¹ School of Pharmaceutical Sciences, Jilin University Fujin Road 1266 Changchun 130021 Jilin China liujp@jlu.edu.cn lipy@jlu.edu.cn thomassk@jlu.edu.cn +86-431-85619803.
² The First Hospital of Jilin University Changchun 130021 Jilin China.
³ Research Center of Natural Drug, Jilin University Changchun 130021 China.

Abstract

With the aim of identifying the active components of Xueshuan Xinmaining Tablet (XXT) and discussing the potential mechanism involved, the relationship between HPLC fingerprints and its blood-activating effect were established by multivariate statistical analysis, including gray relational analysis (GRA) and partial least squares regression analysis (PLSR). Network pharmacology was used to predict the potential mechanism based on the identified active components. GRA and PLSR analysis showed close correlation between the HPLC fingerprints and blood-activating activity, and peaks P1, P3, P11, P15, P22, P34, P36, P38 and P39 might be potential anti-blood stasis components of XXT. The pharmacological verification showed that salvianic acid A (P1), rutin (P3), ginsenoside Rg₁ (P11) and Rb₁ (P22), cinobufagin (P36), and tanshinone I (P38) and IIA (P39) had significant blood-activating effects. Based on these seven active compounds, network pharmacology analysis indicated that the anti-blood stasis effect of XXT might be closely related to TNF, PI3K-Akt and NF-κB signaling pathways. The spectrum-effect relationship of XXT was successfully established in this study. The blood-activating components and the anti-blood stasis mechanism were revealed and predicted. These findings could also be beneficial for an exploration of the active components of TCM.