Investigation of CO2 Extract of Portulaca oleracea for Antioxidant Activity from Raw Material Cultivated in Kazakhstan

Meruyert I Tleubayeva; Raisa M Abdullabekova; Ubaidilla М Datkhayev; Margarita Yu Ishmuratova; Mereke B Alimzhanova; Kaldanay K Kozhanova; Aida M Seitaliyeva; Kairat S Zhakipbekov; Zhanar B Iskakova; Elmira A Serikbayeva; Elena V Flisyuk

doi:10.1155/2022/6478977

Investigation of CO₂ Extract of Portulaca oleracea for Antioxidant Activity from Raw Material Cultivated in Kazakhstan

Int J Biomater. 2022 Apr 22:2022:6478977. doi: 10.1155/2022/6478977. eCollection 2022.

Affiliations

¹ Department of Organization and Management and Economics of Pharmacy and Clinical Pharmacy, Department of Engineering Disciplines, School of Pharmacy, NJSC Karaganda Medical University on the Karaganda Medical University, Almaty 050000, Tole Bi 88 Str, Kazakhstan.
² Departament of Pharmaceutical Disciplines and Chemistry, NJSC Karaganda Medical University, Karaganda 100008, Gogol 40 Str, Kazakhstan.
³ Department of Botany, Karaganda Buketov University, Karaganda 100024, Universitetskaya 28 Str, Kazakhstan.
⁴ Department of Thermal Physics and Technical Physics, Faculty of Physics and Technology, Al-Farabi Kazakh National University, Almaty 050040, Al-Farabi Ave 71, Kazakhstan.
⁵ Department of Fundamental Medicine Faculty of Medicine and Health, Al-Farabi Kazakh National University, Almaty 050040, Al-Farabi Ave 71, Kazakhstan.
⁶ Research Institute of New Chemical Technologies, L.N. Gumilyov Eurasian National University, Nur-Sultan 010000, Satpaev 2 Str, Kazakhstan.
⁷ Departament of Technology of Drugs Forms, St. Petersburg State Chemical and Pharmaceutical University, Petersburg 197376, Popov 14 Str, Kazakhstan.

Abstract

Medicinal plants remain as an important resource in the fight against many diseases, especially in developing countries. Antioxidants are substances capable of delaying, retarding, and preventing the oxidation of lipids or substances that delay or prevent free radical reactions during lipid oxidation. Natural antioxidants such as ascorbic acid, tocopherol, phenolic compounds, and flavonoids are a safe alternative to chemical antioxidants. In present work, results of antioxidant activity of raw materials from the cultivated plant Portulaca oleracea are presented. The extraction time was optimized to 780 minutes; the yield of extractive substances was 1.25% in the production of CO₂ extract under subcritical conditions. For the first time, the antioxidant activity of Portulaca oleracea CO₂ extract was determined by the amperometric method. Gas chromatography-mass spectrometry (GC-MS) chemical analysis of Portulaca oleracea CO₂ extract dissolved in hexane revealed 37 components, including a complex mixture of aldehydes, alkanes, alkenes, esters, diterpenes, steroids, vitamin E, and carbohydrates. The investigation results showed that the Portulaca oleracea CO₂ extract was promising for pharmaceutical, cosmetic, and food industries and had great potential for the prevention and treatment of diseases caused by oxidative stress.