Leucomethylene blue probe detects a broad spectrum of reactive oxygen and nitrogen species

Christopher Cremer; Jean Michél Merkes; Christina L Bub; Dirk Rommel; Frederic W Patureau; Srinivas Banala

doi:10.1039/d1ra06498c

Leucomethylene blue probe detects a broad spectrum of reactive oxygen and nitrogen species

RSC Adv. 2021 Sep 29;11(51):32295-32299. doi: 10.1039/d1ra06498c. eCollection 2021 Sep 27.

Authors

Christopher Cremer¹, Jean Michél Merkes^{1

2}, Christina L Bub¹, Dirk Rommel³, Frederic W Patureau¹, Srinivas Banala^{1

2

4}

Affiliations

¹ Institute of Organic Chemistry, RWTH Aachen University Landoltweg 1 Aachen Germany.
² Institute for Experimental Molecular Imaging, University Clinic, RWTH Aachen Forckenbeckstrasse 55 Germany sbanala@ukaachen.de.
³ DWI-Leibnitz Institute for Interactive Materials and Institute for Technical and Macromolecular Chemistry, RWTH Aachen University Forckenbeckstrasse 50 Aachen Germany.
⁴ Fraunhofer Institute for Digital Medicine MEVIS Max-von-Laue-Str. 2 28359 Bremen Germany.

Abstract

Reactive oxygen and nitrogen species (ROS, RNS) are ubiquitous in biology with a variety of physiological and pathological functions. Here we describe a broad spectrum ROS/RNS detecting fluorogenic probe with red fluorescence emission and up to 100-fold gain. Hence these modified probes are useful for in vivo non-invasive quantification of ROS/RNS.