Downregulation of Lipid Phosphate Phosphatase 3 Correlates With Tumor-Infiltrating Immune Cells in Oral Cancer

Supriya Vishwakarma; Deepti Joshi; Ritu Pandey; Saikat Das; Sramana Mukhopadhyay; Renu Rai; Ritu Singhal; Neelkamal Kapoor; Ashok Kumar

doi:10.7759/cureus.23553

Downregulation of Lipid Phosphate Phosphatase 3 Correlates With Tumor-Infiltrating Immune Cells in Oral Cancer

Cureus. 2022 Mar 27;14(3):e23553. doi: 10.7759/cureus.23553. eCollection 2022 Mar.

Authors

Supriya Vishwakarma¹, Deepti Joshi², Ritu Pandey¹, Saikat Das³, Sramana Mukhopadhyay², Renu Rai⁴, Ritu Singhal⁵, Neelkamal Kapoor², Ashok Kumar¹

Affiliations

¹ Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, IND.
² Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Bhopal, IND.
³ Department of Radiotherapy, All India Institute of Medical Sciences, Bhopal, IND.
⁴ Department of Oncology, Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal, IND.
⁵ Department of Pathology, Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal, IND.

Abstract

Background Sphingosine-1-phosphate (S1P) is a potent oncogenic lipid. Intracellular levels of S1P are tightly regulated by eight S1P-metabolizing enzymes. S1P synthesis is catalyzed by two sphingosine kinases, i.e., sphingosine kinase 1 (SphK1) and sphingosine kinase 2 (SphK2). Five lipid phosphatases (two S1P phosphatases and lipid phosphate phosphatases (LPPs) 1, 2, and 3) reversibly convert S1P back to sphingosine. Previously, we have determined the mRNA expression profile of eight S1P-metabolizing enzymes in tumor tissues and adjacent normal tissues from oral squamous cell carcinoma (OSCC) patients. Except for SphK1, the role of S1P-metabolizing enzymes in OSCC has been poorly studied. Methods We have determined the protein expression of four S1P-metabolizing enzymes (SphK1, SphK2, sphingosine-1-phosphate phosphatase 1 (SGPP1), and lipid phosphate phosphatase 3 (LPP3)) by immunohistochemistry (IHC) in tumor tissues of 46 OSCC patients. Six subjects with non-dysplastic oral mucosa were also included in the study. The immunoreactivity score (IRS) was calculated for each protein in every subject. Further, we determined the associations of expression of S1P-metabolizing enzymes with clinicopathological features of OSCC patients. Results We demonstrate the low IRS for SphK2 and LPP3 in OSCC tumors. Importantly, expression of SphK2 and LPP3 was downregulated in malignant epithelial cells compared to non-malignant mucosa. Further, LPP3 expression negatively correlated with tumor‑node‑metastasis (TNM) staging of patients (r = -0.307, p = 0.043). Importantly, expression of LPP3 in tumors was found to be an independent predictor of perinodal extension (b = -0.440, p = 0.009), lymphovascular invasion (b = -0.614, p < 0.001), lymph node ratio (b = 0.336, p = 0.039), and TNM staging (b = -0.364, p = 0.030). Conclusion Taken together, our data show that expression of SphK2 and LPP3 is decreased compared to normal mucosa. Thus, the S1P signaling pathway could represent a potential therapeutic target.

Keywords: lipid phosphate phosphatase 3; oral squamous cell carcinoma; sphingosine kinase 1; sphingosine kinase 2; sphingosine-1-phosphate.

Grants and funding

This work was funded by the Science and Engineering Research Board (SERB), Government of India’s Scheme for Young Scientist research grant (SB/YS/LS-184/2013), and extramural research grant (EMR/2016/005009) by SERB to AK, ICMR Senior Research Fellowship (ICMR/2019-6836) to SV, and DBT Junior Research Fellowship to RP.